羟苯磺酸钙对慢性马兜铃酸肾病大鼠肾组织CD34和血管性血友病因子表达的影响

目的 观察羟苯磺酸钙对慢性马兜铃酸肾病(CAAN)大鼠肾小管周毛细血管内皮细胞特异抗原CD34和血管性血友病因子(vWF)表达的影响,探讨羟苯磺酸钙改善CAAN大鼠模型肾脏微循环障碍的作用和机制.方法 关木通水煎剂灌胃12周制作马兜铃酸肾病大鼠模型,随机分成未治疗组和治疗组.未治疗组(n=8)予饮用水灌胃4周;治疗组(n=8)予羟苯磺酸钙灌胃4周.另设健康对照组(n=8).实验第16周处死所有大鼠,留取血、尿、肾组织标本行生化、病理及免疫组织化学检查.结果 与未治疗组比较,治疗组肾功能明显改善,肾间质纤维化程度减轻[(38.22±5.17)×103比(69.97±17.69)×103,P＜0.01],CD34阳性表达显著增加[(16.72±4.17)×103比(3.19±1.40)X103,P＜0.01];vWF阳性表达显著减少[(10.16±1.67)×103比(18.66±4.65)×103,P＜0.01)].结论 羟苯磺酸钙能增加CAAN大鼠肾组织CD34的阳性表达,增加肾小管周围毛细血管密度;减少CAAN大鼠肾组织vWF的阳性表达,减少微血栓形成.

Impact of calcium dobesilate on the expression of CD34 and von Willebrand factor in renal tissue of chronic aristolochic acid nephropathy rats

Objective To observe the effects of calcium dobesilate on the expression of CD34 and VOH Willebrand factor(vWF)in peritubular capillary (PTC)in renal tissues of rats with chronic arisolochic acid nephropathy(CAAN)and to explore the probable mechanism concerned.Methotis Sixteen Wistar rats were infused with Caulis aristolochia manshuriensis decoction for 12weeks,then randomly divided into 2 groups.NX group rats(n=8)were infused with distilled water and treatment group(n=8)with calcium dobesilate at the following 4 weeks solely.Control group rats(n=8)were infused with distilled water for the 16 weeks.At week 16,all the rats were sacrificed.Specimens of blood and urine were collected to detect the blood urea nitrogen (BUN),serum creatinie(Scr)and urine protein.HE and Masson staining was used to observe the pathology of the kidney.Immunohistochemistry was used to detect the expression of CD34 and vWF.Results Urinary protein,Scr and BUN in calcium dobesilate treatment group were much lower than those in NX group (P＜0.05).The A value of CD34+ increased significantly in calcium dobesilate treatment group[(16.72±4.17)×103]compared with NX group[(3.19±1.40)×103]at week 16(P＜0.01).The A value of vWF+decreased in calcium dobesilate treatment group[(10.16±1.68)X103]compared with NX group[(18.66±4.65)x103]at week 16(P＜0.01). Conclusion Calcium dobesilate can increase the expression of CD34 and the density of peritubular capillary(PTC)in renal tissues of CAAN rats,and reduce the expression of vWF and the formation of microthrombosis.