c-Jun、Smad7蛋白在大肠腺癌中的表达及临床意义

目的探讨c-Jun、Smad7蛋白在大肠癌中的表达及其对肿瘤发生、发展的影响。方法采用免疫组化方法,分别检测20例正常大肠黏膜、20例大肠腺瘤及65例大肠腺癌中c-Jun、Smad7蛋白表达水平,比较两种蛋白的表达情况。结果 c-Jun蛋白在大肠腺癌组织中的表达率为72.30%,与正常对照组比较差异有统计学意义(P0.05);高分化腺癌与中-低分化腺癌相比,差异有统计学意义(P0.05);TNMⅢ+Ⅳ期表达率显著低于Ⅰ+Ⅱ期(P0.05);Smad7蛋白在大肠腺癌组织中的表达率为89.23%,与对照组比较差异有统计学意义(P0.05);中-低分化者、有淋巴结转移者与高分化者、无淋巴结转移者比较差异均有统计学意义(P均0.05)。c-Jun蛋白与Smad7蛋白在大肠腺癌中的表达水平呈正相关(P0.05,r s=0.3460)。结论 c-Jun、Smad7蛋白在大肠腺癌的发生、发展整个过程的不同阶段具有促进作用,对于临床诊断大肠癌及判断病情、预后有重要意义。

The expression and clinical significance of c-Jun and Smad7 proteins in colorectal adenocarcinoma

Objective To study the expression of c-Jun, Smad7 proteins and the effect on colorectal adenocarcino- ma. Methods The expression of c-Jun, Smad7 proteins were detected by the immunohistochemical （IHC） staining technique in colorectal adenocarcinoma （65 cases） , normal colorectal mucosa （20 cases） and colorctal adenoma （20 cases） , the expression of two proteins were compared, respectively. Results The expression of c-Jun protein in color- ectal adenocarcinoma tissues was 72.30% , and had statistical significance compared with the normal control group （ P 〈 0.05）. There was significant difference between well-differentiated and poorly differentiated colorectal adenocarcinoma （P 〈 0.05）. The expression of c-Jun in TNM m ＋ rv was significantly lower than that of TNM I ＋ II （P 〈 0.05）. The expression of Smad7 protein was 89.23% and had statistically significant compared with normal control group （P 〈 O. 05）. Meanwhile the Smad7 protein in patients with poorly differentiated and with lymph node metastasis was higher than that in patients with well-differentiated and without lymph node metastasis （P 〈 0.05）. There was a postive corre- lation between c-Jun protein and Smad7 protein in colorectal adenocarcinoma （P 〈 0.05, r, = 0. 3460）. Conclusion The c-Jun and Smad7 proteins could promote the development of colorectal adenocarcinoma and could be play important roles in clinical diagnosis and prognosis.