Oxotremorine acts as a partial nicotinic agonist on cultured chick myotubes

Oxotremorine, a compound widely used as a muscarinic agonist, was found to inhibit binding of 125I-alpha-bungarotoxin to chick myotube nicotinic acetylcholine receptors, IC50 = 79 +/- 5 microM. Oxotremorine also induced a d-tubocurarine sensitive influx of 86Rb in the myotubes (EC50 = 50 +/- 15 microM). Comparative ion-flux studies with carbachol suggested a partial agonist mode of action of oxotremorine.