重组人促红细胞生成素抑制大鼠颈脊髓亚急性压迫性损伤中细胞凋亡的实验研究

目的  研究重组人促红细胞生成素(rhEPO)对大鼠颈脊髓亚急性压迫性损伤的治疗效果。方法  80只Wistar大鼠随机分为4组，假手术组（A组，n=5）仅行椎板切除，余大鼠用后路渐进性压迫法造成C5节段脊髓压迫性损伤。造模成功后，生理盐水对照组（B组，n=25）静脉给予生理盐水1mL／d，小剂量治疗组（C组,n=25）和大剂量治疗组（D组，n=25）分别静脉给予rhEPO 500u/(kg·d)、5000u/(kg·d)；损伤后分别于3、7、14、21、28d用BBB评分评价四肢运动功能；免疫组化法检测受压颈髓节段EPO和EPO-R的分布及数量；各时间点处死模型，脊髓标本进行TUNEL染色和流式细胞仪定量检测细胞凋亡情况。结果   EPO-R随颈脊髓亚急性压迫性损伤渐重呈反应性增多，C、D组的各时间点BBB评分明显高于B组（P＜0.01）；TUNEL染色和流式细胞仪检测受压脊髓细胞凋亡结果显示：在21d时，rhEPO最能有效抑制脊髓运动神经元细胞的凋亡（P＜0.01），小剂量rhEPO维持治疗给药剂量效果好。结论  颈脊髓亚急性压迫性损伤后给予rhEPO可减轻脊髓的运动神经元细胞的凋亡，促进脊髓运动功能恢复，且小剂量rhEPO维持治疗即可取得良好效果。

Inhibitory effect of recombinant human erythropoietin on motor neuron apoptosis during cervical chronic spinal cord compression in rats

Objective To investigate effects of recombinant human erythropoietin (rhEPO) on chronic cervical spinal cord compression in rats. Methods 80 Wistar rats were randomly separated into 4 groups. Control rats (group A) received sham operations (n =5) and were executed by laminectomy. Experimental rats were subjected to compression injury in the C5 segment of the spinal cord by posterior progressive compression and subsequently subdivided into 3 groups. Group B (n =25) was treated with 1mL saline by IV. Groups C...