中医"肾应冬"调控机制与褪黑素受体关系的研究

目的通过观察性腺轴褪黑素受体的季节性变化,从而深入揭示“肾应冬”的调控机制。方法采用放射性配基饱和法,检测雄性SD大鼠性腺轴褪黑素受体的最大结合量(Bm ax)和解离常数(Kd值)的冬夏变化。结果在正常生理条件下,下丘脑(P<0.05)和垂体(P<0.05)Bm ax值均表现为冬高夏低。下丘脑(P<0.05)和垂体(P<0.01)Kd值均表现为冬高夏低。摘除松果腺后,冬季手术组下丘脑(P<0.01)、垂体(P<0.01)Bm ax值都明显高于正常组,夏季手术组下丘脑(P<0.01)Bm ax值明显高于正常组。冬、夏手术组下丘脑(P<0.01)、垂体(P<0.01)Kd值都明显高于相应的正常组。而睾丸的褪黑素受体的Bm ax值和Kd值因含量极少而无法测出。结论“肾应冬”调控机制与性腺轴褪黑素受体的季节性变化有关,松果腺在此过程中起了重要的高位调节作用。

Relationship between regulatory mechanism of "kidney corresponding with winter" and melatonin receptor

Objective To study the regulatory mechanism of "kidney corresponding with winter" through the observation of the seasonal changes of melatonin receptor.Method The radioligand saturation method was used to detect the changes of maximal combination(Bmax) value and dissociation constant(Kd value) of melatonin receptor of sex gland axis in winter and in summer in male SD rats.Results Bmax values of melatonin receptor of hypothalamus and pituitary in the normal group were both high in winter and low in summer(P<0.05,P<0.05),while Kd values of melatonin receptor of them were also high in winter and low in summer(P<0.05,P<0.01).In the pineal gland removed group,Bmax values of melatonin receptor of hypothalamus and pituitary were higher than those of the normal group in winter(P<0.01,P<0.01),and Bmax value of melatonin receptor of hypothalamus was higher than that of the normal group in summer(P<0.01).Kd values of melatonin receptor of hypothalamus and pituitary in the pineal gland removed group were higher than those in the normal group in winter and in summer(P<0.01,P<0.01).Bmax value and Kd value of melatonin receptor could not be detected in testicle.Conclusion The regulatory mechanism of "kidney corresponding with winter" is related with the seasonal changes of melatonin receptor,and the pineal gland plays an important role in the process of regulation.