NDR2蛋白在甲状腺乳头状腺癌及正常甲状腺组织中的表达及意义

目的:探讨人正常甲状腺及甲状腺癌组织中NDR2蛋白的表达及临床意义。方法:随机收集正常甲状腺及乳头状甲状腺癌病理标本,采用小鼠抗NDR2单克隆抗体的免疫组织化学ABC法,研究NDR2蛋白在人正常甲状腺及甲状腺乳头状腺癌中的表达,并结合图像分析比较NDR2蛋白在正常甲状腺组织与癌组织中含量的变化。结果:人正常甲状腺滤泡上皮细胞胞浆均呈现较强的阳性NDR2免疫反应,胞核中未见有阳性物质表达。乳头状腺癌细胞胞浆免疫反应阳性很弱,阳性率为23.3％,胞核呈阴性。正常甲状腺组NDR2免疫反应阳性率与甲状腺乳头状腺癌组差异有显著性(P<0.05)。图像分析结果显示,正常甲状腺组NDR2蛋白相对含量与甲状腺乳头状腺癌组差异有显著性(P<0.05)。结论:NDR2在正常甲状腺组织及甲状腺乳头状腺癌中表达差异有极显著性,推测NDR2可能参与了乳头状甲状腺癌的发病机制。进一步研究NDR2蛋白的功能有重要意义。

Expression and Significance of NDR2 in Thyroid Pilliary Adenocarcinoma and Normal Thyroid Tissue

Objectlve To explore the possible expression and function of NDR2 protein in human normal thyroid and papillary thyroid cancer. Methods Immunohistochemical ABC method and image analysis were used to investigate the expression and content of NDR2 in human normal thyroid and carcinoma biopsies. Results The positive cytoplasm immunostaining were observed in all normal thyroid follicular epithelia cells, but the nuclei were negative. The cytoplasm of papillary thyroid carcinoma cells were showed weaker immunoreactivity with the immuno-negative nuclei, and the positive rate was 23.3 % . There was significant difference of staining positive rates and intensity between normal thyroid and papillary thyroid carcinoma(P < 0.05) . Conclusions There is significant difference of NDR2 between normal thyroid and papillary thyroid cancer. NDR2 may take part in the pathogenesis of papillary thyroid carcinoma. It is important to further study NDR2.