缺血心肌应用bFGF后心肌中VEGF的表达

目的　探讨心肌内注射碱性成纤维生长因子 (basicfibroblastgrowthfactor ,bFGF)对急性心肌梗死 (myocardialinfarction ,MI)血管内皮生长因子 (vascularendothelialgrowthfactor ,VEGF)的表达作用。方法　 2 4只犬建立急性MI模型后随机分成对照组 (MI区注射生理盐水 15ml)和实验组 (MI区注射 5 0mgbFGF与生理盐水的混合液 15ml) ;每组观察 4个不同的时间点 (术后第 1、3、10、17天 )。各组动物分别在处死前应用敏感编码技术 (sensitivityencodedtechnique ,SENSE)行磁共振电影成像 (cinemagneticresonanceimag ing ,cine -MRI)。免疫组织化学方法检测VEGF的表达。结果　心肌缺血区对照组VEGF的表达增多 ;实验组左心室射血分数 (leftventricularejectionfraction ,LVEF)自第 10天明显增加 (第 10天 :对照组 2 4.0 9± 3 .3 2、实验组 45 .71± 6.2 7;第 17天 :对照组 3 1.46± 4.60、实验组 5 3 .46± 5 .2 4;单位 :%)。结论　局部心肌内注射bFGF有促进MI区域VEGF的表达及提高左心室功能的作用

The Effect of bFGF on the Expression of VEGF in Ischeamic Myocardium

Objective To evaluate the effect of intramyocardial administration of basic fibroblast growth factor on the expression of VEGF in infarcted myocardium and on cardiac function. Methods Twenty-four mongrel dogs were randomized into control group and therapeutic group. Acute myocardial infarction was made by ligation of the left anterior descending coronary artery distal to its first diagonal branch. After coronary artery was occlused,50 microgram of basic fibroblast growth factor in 15 ml of saline was injected into the infarcted myocardium and its border zone in therapeutic group, whereas 15 ml saline alone was used in control dogs.Every 3 dogs in each group were studied on the 1st day,the 3rd day ,the 10th day and the 17th day after surgery respectively. With sensitivity encoded technique,cine MR was performed on each dog before euthanasia to evaluate cardiac function. The expression of VEGF was evaluated by immunohistochemical study with the antibody of VEGF.Results VEGF expressed more in therapeutic group. In therapeutic group,LVEF improved markedly since the 10th day after injection of bFGF. Conclusion Intramyocardial administration of basic fibroblast growth factor is useful for increase in the expression of VEGF and improvement in left ventricular function in acute myocardial infarction.