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灯盏花种质资源遗传关系的RAPD分析
引用本文:杨生超,李永芳,赵峥,文国松,李云,刘有贵,杨永建,萧凤回. 灯盏花种质资源遗传关系的RAPD分析[J]. 中国中药杂志, 2008, 33(13): 1532-1535
作者姓名:杨生超  李永芳  赵峥  文国松  李云  刘有贵  杨永建  萧凤回
作者单位:1. 云南农业大学,中药材研究所,云南省中药材规范化种植技术指导中心,云南,昆明,650201
2. 云南生物谷灯盏花药业有限公司,云南,昆明,650224
基金项目:国家"十五"科技攻关项目 , 云南省"十一五"社会发展攻关项目
摘    要:目的:研究灯盏花种质资源间的遗传关系。方法:对采自云南、贵州和四川等地的37份灯盏花种质资源进行RAPD遗传多样性分析和NTSYS2聚类分析。结果:筛选出的10个引物在37份种质资源中共产生107条DNA片段,其中94条带具有遗传多态性,约占87.85%。遗传距离为0.36时,可以把灯盏花分为三大类,第一大类包括采自滇东南的弥勒、丘北、泸西、个旧、砚山等地的9份种质资源;第二大类包括采自滇西北的古城、香格里拉的6份,以及滇东南丘北和弥勒各1份,共8份种质资源;第三大类包括采自滇中、滇东、滇东北、四川和贵州等地的20份种质资源。结论:灯盏花种质资源遗传多样性丰富,遗传关系与其采集地的地理分布距离密切相关。

关 键 词:灯盏花  种质资源  遗传关系  RAPD
收稿时间:2007-07-27

RAPD analysis on genetic relationships of Erigeron breviscapus germplasm resources
YANG Sheng-chao; LI Yong-fang; ZHAO Zheng; WEN Guo-song; LI Yun; LIU You-gui; YANG Yong-jian; XIAO Feng-hui. RAPD analysis on genetic relationships of Erigeron breviscapus germplasm resources[J]. China Journal of Chinese Materia Medica, 2008, 33(13): 1532-1535
Authors:YANG Sheng-chao   LI Yong-fang   ZHAO Zheng   WEN Guo-song   LI Yun   LIU You-gui   YANG Yong-jian   XIAO Feng-hui
Affiliation:Institute of Chinese Medicinal Materials of Yunnan Agricultural University/Yunnan Provincial Center of Chinese Medicinal Materials' Good Agriculture Practice Technology, Kunming 650201, China.
Abstract:OBJECTIVE: To investigate the genetic relationships of Erigeron breviscapus at the molecular biology level. METHOD: Thirty seven germplasm resources of E. breviscapus which collected from Yunnan, Sichuang and Guizhou province in 2005 were analyzed by Random amplified polymorphic DNA (RAPD) and cluster analysis based on NTSYS2. RESULT: A total of 10 primers were screened, and 107 bands were amplified, among which 94 (87.85%) bands were found to be polymorphic. Thirty seven germplasm resources of E. breviscapus were clustered into 3 groups at genetic distance 0.36, the I group include in 9 germplasm resources collected from Mile, Qiubei, Luxi, Gejiu, and Yanshan of south east of Yunnan province; the II group included 8 germplasm resources collected from Gucheng, and shangrila of north west of Yunnan province, and Mile and Qiubei of south east of Yunnan province; the III group included in 20 germplasm resources collected from the center of Yunnan province, north east of Yunnan province, Sichuan province, and Guizhou province. CONCLUSION: There were abundant genetic diversity in the germplasm resources of E. breviscapus, and the genetic relationships are closely related to geographical distance where they were collected.
Keywords:Erigeron breviscapus   germplasm resources   genetic relationships   RAPD
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