| 特定序列寡核苷酸对人骨髓间充质干细胞体外扩增及成骨分化的影响 |
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| 引用本文： | 刘春秀,满大鹏,杜晓岩,康宁,董平,肖苒,王丽颖.特定序列寡核苷酸对人骨髓间充质干细胞体外扩增及成骨分化的影响[J].组织工程与重建外科,2013,9(1):1-4. |
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| 作者姓名： | 刘春秀 满大鹏 杜晓岩 康宁 董平 肖苒 王丽颖 |
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| 作者单位： | 刘春秀 (154004,黑龙江省佳木斯市 佳木斯大学附属第二医院、口腔医院整形外科)；满大鹏 (154004,黑龙江省佳木斯市 佳木斯大学附属第二医院、口腔医院整形外科)；杜晓岩 (154004,黑龙江省佳木斯市 佳木斯大学附属第二医院、口腔医院整形外科)； 康宁 (100041,北京市 中国医学科学院整形外科医院研究中心)；董平 (100041,北京市 中国医学科学院整形外科医院研究中心)； 肖苒 (100041,北京市 中国医学科学院整形外科医院研究中心)； 王丽颖 (130021,吉林省长春市 吉林大学基础医学院分子生物学教研室)； |
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| 基金项目： | 佳木斯大学重大培育基金项目(SZP2001-001) |
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| 摘 要： | 目的探讨特定序列人工合成的寡核苷酸（Oligodeoxynucleotide，ODN）MT01对人骨髓间充质干细胞（Human bone marrow mesenehymal stem cells．hBMSCs）增殖及成骨分化的影响。方法采用Ficoll梯度密度离心法分离培养hBMSCs并进行鉴定。以1μg／mL的ODN MT01处理hBMSCs，细胞计数试剂盒检测细胞增殖情况，碱性磷酸酶试剂盒检测成骨分化情况。结果经1μg／mL的ODN MT01处理的hBMSCs体外培养，第3、4、5、6、7天hBMSCs增殖明显；成骨诱导的第4、14、21天．碱性磷酸酶表达明显增加。结论特定浓度人工合成ODN MT01能够在体外促进hBMSCs的扩增及成骨分化。
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| 关 键 词： | 骨髓问充质干细胞 寡核苷酸 增殖 成骨分化 |
The Influences of Specific Synthetic Oligodeoxynucleotide on the Proliferation and Osteogenic Differentiation of hBMSCs |
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| Authors: | LIU Chunxiu MAN Dapeng DU Xiaoyan KANG Ning DONG Ping XIAO Ran WANG Liying |
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| Institution: | 1 Department of Orthopaedics,Hospital of Stomatology,Jiamusi University,Jiamusi 154004,China;2 Research Center,Plastic Surgery Hospital,Chinese Academy of Medical Sciences,Beijing 100041,China;3 Department of Molecular Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China. |
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| Abstract: | Objective To investigate the influences of specific synthetic oligodeoxynucleotides （ODN） on the proliferation and osteogenie differentiation of hBMSCs. Methods The hBMSCs were isolated via Ficoll density gradient centrifugation and then identified after amplification. Then the hBMSCs were cultured by synthetic ODN MT01 with a eentrifugation of 1 μg/mL. Proliferation of hBMSCs were measured using cell counting kit, and the influences on osteogenic differentiation were detected by alkaline phosphatase kit. Results The growth of hBMSCs was promoted by ODN MT01 with a eentrifugation of 1μg/ml on the 3, 4, 5, 6, 7 day, and the ATP activity of hBMSCs was also promoted on the 4, 14, 21 day after induced. Conclusion Certain concentration of synthetic ODN MTO1 can promote the growth and osteogenic differentiation of hBMSCs in vitro. |
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| Keywords: | Bone marrow mesenchymal stem cells Oligodeoxynucleotide Proliferation Osteogenic differentiation |
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