Synthesis of glutathione C60 derivative and its protective effect on hydrogen peroxide-induced apoptosis in rat pheochromocytoma cells |
| |
Authors: | Hu Zhen Liu Shenghong Wei Yen Tong Etang Cao Fei Guan Wenchao |
| |
Institution: | Department of Chemistry, Huazhong University of Science and Technology, Wuhan 430074, China. |
| |
Abstract: | Oxidized glutathione C(60) derivative has been synthesized and characterized in our research. As a novel derivative of C(60), the glutathione C(60) derivative is soluble in dimethylsulfoxide, dimethylformamide and dimethylacetamide. Rat pheochromocytoma (PC12) cells are treated with hydrogen peroxide and underwent cytotoxicity; apoptotic death is determined by MTT assay, flow cytometry analysis, PI/Hoechst 33342 staining and glutathione assay. The results suggest that glutathione C(60) derivative has the potential to prevent oxidative stress-induced cell death without evident toxicity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|