| 巨噬细胞内吡喹酮的HPLC法测定 |
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| 引用本文： | 邹永华,丁劲松,何世英,张龙贵,马宁. 巨噬细胞内吡喹酮的HPLC法测定[J]. 中国医药工业杂志, 2012, 43(9): 773-775 |
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| 作者姓名： | 邹永华 丁劲松 何世英 张龙贵 马宁 |
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| 作者单位： | 1. 中南大学药学院,湖南长沙,410013
2. 长沙医学院,湖南长沙,410219 |
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| 基金项目： | 湖南省教育厅(09A011)重点项目 |
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| 摘 要： | 建立了高效液相色谱法测定体外小鼠腹腔巨噬细胞摄取的吡喹酮含量。采用Kromasil 100-5 C18色谱柱,流动相为甲醇-水(67︰33),检测波长264 nm。吡喹酮在2～40 g/ml范围内线性关系良好。低、中、高浓度质控样品的方法回收率为(98.24±2.99)%、(104.49±4.27)%和(105.13±6.05)%,RSD小于10.6%。
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| 关 键 词： | 吡喹酮 长循环纳米粒 巨噬细胞 高效液相色谱 测定 |
Determination of Praziquantel in Macrophages by HPLC |
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| ZOU Yonghua , DING Jinsong , HE Shiying , ZHANG Longgui , MA Ning. Determination of Praziquantel in Macrophages by HPLC[J]. , 2012, 43(9): 773-775 |
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| Authors: | ZOU Yonghua DING Jinsong HE Shiying ZHANG Longgui MA Ning |
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| Affiliation: | 1.School of Pharmaceutical Sciences,Central South University,Changsha 410013;2.Changsha Medical University,Changsha 410219) |
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| Abstract: | An HPLC method was established for the determination of praziquantel in mouse peritoneal macrophage in vitro.A Kromasil 100-5 C18 column was used,with the mobile phase of methanol-water(67︰33) at the detection wavelength of 264 nm.The calibration curve was linear in the range of 2-40 μg/ml.The method recoveries of low-,middle-and high-concentration quality control samples were(98.24±2.99)%,(104.49±4.27)% and(105.13± 6.05)%,with RSDs less than 10.6%. |
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| Keywords: | praziquantel long circulating nanoparticle macrophage HPLC determination |
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