| 反相高效液相色谱法测定血清中头孢他美浓度 |
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| 引用本文： | 卢华,卢来春.反相高效液相色谱法测定血清中头孢他美浓度[J].中国药房,2002,13(6):334-336. |
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| 作者姓名： | 卢华 卢来春 |
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| 作者单位： | 1. 山东省莱芜市寨里医院,莱芜市,271115
2. 第三军医大学新桥医院药剂科,重庆市,400037 |
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| 摘 要： | 目的 :以反相高效液相色谱法测定血清中头孢他美浓度。方法 :血清样品经甲醇沉淀蛋白 ,10000r/min离心15min后取上清液直接进样。色谱分析条件 :hypersilBDSC18(4 6mm×150mm ,5μm) ;流动相 :甲醇∶水=28∶72(V/V ,pH2 8) ;流速 :1 0ml/min ;柱温 :室温 ;检测波长 :262nm。结果 :血清中头孢他美在0 53～13 25μg/ml浓度范围内线性关系良好 ,最低检出浓度为0 25μg/ml,平均方法回收率为100 84 % ,日内RSD≤4 42 % ,日间RSD≤4 17 %。结论 :本法简便、灵敏、准确可靠 ,可用于头孢他美的体内药代动力学研究。
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| 关 键 词： | 反相高效液相色谱法 头孢他美 血清 |
| 文章编号： | 1001-0408(2002)06-0334-03 |
| 修稿时间： | 2001年8月13日 |
Determination of Cefetamet in Dog Serum by RP- HPLC |
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| LU Hua.Determination of Cefetamet in Dog Serum by RP- HPLC[J].China Pharmacy,2002,13(6):334-336. |
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| Authors: | LU Hua |
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| Abstract: | OBJECTIVE:A RP-HPLC method was developed for determination of cefetamet in serum METHODS:The serum samples were detected on a hypersil BDS C18 column after preciptating protein with methyl alcohol and being centrifuged at a speed of 10 000 r/min The mobile phase was methyl alcohol-water=28∶72(V/V),pH=2 8,the flow rate was 1 0ml/min and the detection wavelength 262nm RESULTS:The methods showed good linearity in a concentration range of 0 53～13 25μg/ml The minimun detactable concentration of cefetamet in serum was 0 25μg/ml,the mean recovery was 100 84%,the within-day RSD was less than 4 42% and the between-day RSD was less than 4 17% CONCLUSION:The method is simple,sensitive and accurate,and may be applied to the pharmacokinetics study of cefetamet in vivo |
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| Keywords: | RP-HPLC cefetamet serum |
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