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| HPLC法测定复方环吡酮胺溶液中有效成分的含量 | |
| 引用本文： | 戴博,张华峰,涂秀杰,夏莹.HPLC法测定复方环吡酮胺溶液中有效成分的含量[J].中国药事,2010,24(10):997-999. |
| 作者姓名： | 戴博 张华峰 涂秀杰 夏莹 |
| 作者单位： | 1. 空军总医院,北京,100142 2. 河北北方学院 3. 内蒙古医学院 |
| 摘 要： | 目的建立HPLC法同时测定复方环吡酮胺溶液中苯佐卡因和环吡酮胺两组分的含量。方法采用美国Agilent ZORBAX SB-C18色谱柱,以甲醇-乙腈-水-冰醋酸（15∶35∶50∶0.3）为流动相。检测波长为300nm,流速为1mL.min-1。结果苯佐卡因和环吡酮胺在15min内完全分离,两者在10～120mg.L-1范围内线性关系良好,平均回收率分别为101.15%和100.70%,相对标准偏差（RSD）均小于2%（n=9）;日内精密度和日间精密度（RSD）均小于2%（n=5）。结论本法准确、可靠、重复性好,可有效控制环吡酮胺溶液的质量。 |
| 关 键 词： | HPLC 复方环吡酮胺溶液 苯佐卡因 环吡酮胺 |
Determination of Benzocain and Cidopirox Olamine in Compound Cidopirox Olamine Solution |
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| Dai Bo,Zhang Huafeng,Tu Xiujie,Xia Ying.Determination of Benzocain and Cidopirox Olamine in Compound Cidopirox Olamine Solution[J].Chinese Pharmaceutical Affairs,2010,24(10):997-999. | |
| Authors: | Dai Bo Zhang Huafeng Tu Xiujie Xia Ying |
| Institution: | (Air Force General Hospital,Beijing 100142;1Hebei North University;2Inner Mongolia Medical College) |
| Abstract: | Objective To establish a method for simultaneous determination of benzocain and cidopirox olamine in Compound Cidopirox Olamine Solution.Methods The sample was separated on Agilent ZORBAX SB-C18 column,with the mobile phase consisting of methanol-acetonitrile-water-acetic acid（15∶35∶50∶0.3）,the flow rate of 1.0mL·min-1,and the detection wavelength at 300nm.Results Within the range of 10～120μg·mL-1 of benzocain and cidopirox olamine,the calibration curve was linear.The mean recovery rate（%） of benzocain was 101.15（RSD=1.53%,n=9）,and the mean recovery rate（%） of cidopirox olamine was 100.70（RSD=1.64%,n=9）.Conclusion The method is accurate,reliable,stable and reproducible.It can be used for determination of Compound Cidopirox Olamine Solution |
| Keywords: | HPLC |
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