人芽囊原虫粪便检查与形态观察

目的了解人芽囊原虫的粪便检查方法和光镜下的形态特点.方法对就诊病人新鲜粪便,用生理盐水涂片、碘染色和吉氏染色三种方法制作玻片标本,光镜下观察人芽囊原虫的形态特征. 结果1495例就诊病人,查出162例人芽囊原虫感染,感染率为10.84%;三种方法可查见人芽囊原虫滋养体期的空泡型和颗粒型,检出率相近,生理盐水涂片偶见阿米巴样型,碘液染色可见包囊期.结论广西人芽囊原虫感染较高,生理盐水涂片和碘染色方法适用于人芽囊原虫的粪便检查,本观察图片可为临床检验提供参考.

Stool examination and morphological observation of Blastacystis hominis

Objects To understand the methods of the stool examination and morphology feature of Blastacystis hominis. Methods The fresh fecal specimen of patients were examined by direct smear, iodine stained and giemsa stained method under light microscope. Results It was observed that out of the 1 495 outpatients,162 were infected,with an infection rate of 10.84%. The Vacuolated form and Granular form of B.hominis can be easily identified and the Ameba form be seen occasionaly on direct normal saline smear, the cyst can be identified after stained with iodine. Conclusion The prevalence of B.hominis in Guangxi area is high. Direct smear and iodine stained examination of faeces is adequate for detection of B.hominis infection and the photographs of B.hominis can serve as reference for clinical diagnosis.