刺五加叶皂苷单体Sc3对培养大鼠心肌细胞自发性搏动及动作电位的影响

 目的：观察刺五加皂苷单体Sc₃(Acanthopanax Senticoside C₃)对培养的Wistar大鼠乳鼠心肌细胞自发性搏动及动作电位电参数的影响。方法：将50～800μｇ／ml Sc₃，分别加入到培养4～6d后的Wistar大鼠乳鼠心肌细胞的培养液中，依次记录加药前、后自发性搏动及动作电位电参数的变化。结果：Sc₃可剂量依赖性地抑制被培养的Wistar大鼠乳鼠心肌细胞的自发性搏动及动作电位的电参数，其作用与0.4μｇ／ml尼莫地平相似，而该作用被80μｇ／ml Ca^２+所反转。结论：Sc₃能够阻滞Ca^２+通道。

Influence of Acanthopanax senticosides C３on spontaneous contractility and action potential of cultured rat myocardiocytes

OBJECTIVETo observe the influence of Sc₃(Acanthopanax senticoside C₃)on the spontaneous conrtractility and the electricalparameters of action potential of the cultured neonatal Wistar rat myocardicytesMETHODS:50～800 μg/ml Sc₃ was added into the culture fluid of the neonatal Wistar rat myocardiocytes 4～6 days after culture,and then the changes of the spontaneous contractility and the electrical parameters of action potential recorded after and before administration.RESULTS:Sc₃ inhibited the spontaneous contractility and the electrical parameters of action potential of the cultured neonatal Wistar rat myocardiocytes dose dependently,which was similar to that of 0.4 μg/ml nimodipine,however,could be reversed by 80 μg/ml Ca²⁺.CONCLUSION:Sc₃ could block calcium channel.