佐剂关节炎大鼠脊神经节中核因子kappa B表达的研究

目的：研究核因子kappa B(NF-κB)在佐剂关节炎（AA）大鼠脊神经节（L1-L4）中的表达,以探讨类风湿关节炎（RA）发病的神经-内分泌—免疫学机制。 方法： 用免疫组织化学法检测脊神经节中NF-κB蛋白表达，用Western blotting法分析脊神经节细胞胞核蛋白中NF-κB含量改变。 结果： 在AA大鼠L1-L4脊神经节细胞中，NF-κB蛋白表达明显高于正常对照组（P<001）,且活化的NF-κB（胞核蛋白中NF-κB）含量明显高于正常对照组（P<001）。细胞核内P65蛋白表达量与关节肿胀程度呈正相关。结论： 脊神经节中NF-κB活化可能参与RA发病机制。

Expression of nuclear factor kappa B in the spinal ganglions of adjuvant arthritis rats

AIM: To study the expression of nuclear factor kappa B (NF-κB) in the L1-L4 spinal ganglions of adjuvant arthritis (AA) rats, to explore the neuroendocrinoimmunological mechanism of rheumatoid arthritis (RA). METHODS: The expression of the NF-κB protein was detected by immunohistochemistry method and the alteration of the active NF-κB protein (nuclear protein) was investigated by Western blotting analysis. RESULTS: The expression of NF-κB protein in L1-L4 spinal ganglions of AA rats was much higher than that in the healthy controls (P<001), and the active NF-κB content in L1-L4 spinal ganglions in AA rats was much higher than that in the healthy controls (P<001). CONCLUSION: The active NF-κB protein in the spinal ganglions may be involved in the pathogenesis of the rheumatoid arthritis.