胃经穴位电针对胃运动的调节及对星形胶质细胞GFAP、小胶质细胞OX42表达的影响

目的探讨电针刺激胃经穴对胃运动的调节作用及其作用机制，明确星形胶质细胞和小胶质细胞在其中的作用。方法采用同步实验，在电针足三里穴、上巨虚穴及非经非穴等穴位的情况下，应用免疫组织化学染色的方法，通过对胶质原纤维酸性蛋白（glial fibrillary acidic protien，GFAP）和OX42的ABC法染色，观察延髓内脏带（medullary visceral zone，MVZ）的迷走复合体（dorsalvagalcomple，DVC）中与疼痛密切相关的神经星形胶质细胞（astrocyte，AS）和小胶质细胞（microglia cells）的变化，同时采用浆膜法检测胃电的变化。结果电针刺激足三里穴，在延髓内脏带中的迷走复合体中神经胶质细胞变化及胃电的变化情况均较明显，与电针刺激胃经其他穴位存在显著差异。结论胃经穴位电针对胃运动具有调节作用。这种调节作用的实现，与疼痛作用密切相关。

Effects of electroacupuncture at the points of Stomach meridian on the gastric motion and its influence on the expressions of GFAP,OX42 in dorsal vagal comple of rats

Objective To explore the regulating effects and mechanism of gastric motility by electroaeupuneture at the points of Stomach meridian. Meanwhile, to detect effects of astrosyte （AS） and microglia cells on the gastric motility. Methods Using synchronized experimental methods, the points Zusanli （ST36）, Shangjuxu （ST37）, and some non-aeupoint were acupunetured by eleetroacupuneture. As and microglia cells changes were observed, which existed in dorsal vagal complex（DVC） of medullary visceral zone （MVZ） were closely related with pain. Immunonhistochemistry assay was introduced to stain glial fibrillary acidic protein （GFAP） and OX42 by ABC. Using series coating method, gastroelectrie activity changes were recorded. Results Compared with aeupunctured at other acupoint in Stomach meridian, aeupunctured at Zusanli （ST36） could significantly change the microglia cells in DVC and gastro-electric activity. Conclusion Electroacupuncture at the points of Stomach meridian could modulate gastric motility and this modulating effects is closely related with pain.