内侧前脑束不同部位注射6-羟多巴胺建立帕金森病大鼠模型的对比性研究

目的 建立简单、易行、成功率高的制作帕金森病大鼠模型的方法 。方法 注射同等剂量的6-羟多巴胺于内侧前脑束不同部位（第1组注射位点：位点1：前囟后1.8mm,右侧2.0mm,背腹8.0mm;位点2：前囟后1.8mm,右侧3.0mm,背腹7.5mm。第2组注射位点：位点1：前囟后1.8mm,右侧2.5mm,背腹8.0mm;位点2：前囟后1.8mm,右侧2.5mm,背腹7.5mm）,造成黑质多巴胺能神经元的损坏,运用阿朴吗啡诱发旋转试验及免疫组化检验模型是否成功。比较两组注射位点的成功率。结果 采用第1组注射位点的成功率为51%,第2组注射位点的成功率为77%,显著高于第1组（P〈0.01）。结论 采用第2组注射位点制作帕金森病大鼠模型成功率高、易行。

Study on Comparing Different Means of Injection 6-HODA into Medial Fore Brain Bundles and Establish the Rat Model with Parkinson Disease

Objective To establish a simple and effective rat model of Parkinson disease(PD).Methods In two groups,6-HODA was injected into medial forebrain bundles at the two coordinates on the right sides at the same dosage.In first group,two burr holes were made with a dental drill,two coordinates are 2.0 mm lateral to the midline,8.0 mm in vertically,and-1.8 mm anterior-posterior at the zero point to the bregma;3.0 mm lateral to the midline,7.5 mm in vertically,and-1.8 mm anterior-posterior at the zero point to the bregma.In second group,one burr hole was made,two coordinates are 2.5 mm lateral to the midline,8.0 mm and 7.5 mm in vertically,and-1.8 mm anterior-posterior at the zero point to the bregma.After 6 weeks,apomorphine-induced rotation test and immunohistochemiscal test were performed to examine the disease progress in this rat model.Results In group 1,51%of them were proved to be successful by apomorphine induction.In group 2,the successful ratio is 77%.There was significant difference(P< 0.01)between the two groups.Conclusion This rat model in PD can be created in high successful ratio by two injection points with 6-HODA,which are 2.5 mm lateral to the midline,8.0 mm and 7.5 mm in vertically,and-1.8 mm anterior-posterior at the zero point to the bregma.