HPLC法测定人血浆中盐酸特拉唑嗪的浓度

目的:建立以反相高效液相色谱法测定人血浆中盐酸特拉唑嗪浓度的方法。方法:血样采用二氯甲烷-乙醚(2:3)提取后测定,其中色谱柱为Diamonsil C18,流动相为0·05mol·L-1(pH4·8)磷酸二氢钾缓冲液-甲醇-乙腈(61:25:14),流速为1·0mL·min-1,荧光检测器激发波长为334nm,发射波长为385nm。结果:盐酸特拉唑嗪检测浓度在0·25～64ng·mL-1范围内线性关系良好(r=0·9995),最低检测限为0·1ng·mL-1;平均提取回收率为84·0%,平均方法回收率为100·8%;日内RSD≤3·4,日间RSD≤3·0。结论:本方法简便、准确、灵敏,可用于人血浆中盐酸特拉唑嗪的临床药动学研究和血药浓度检测。

Determination of Terazosin Hydrochloride in Human Plasma by HPLC

OBJECTIVE:To establish a RP-HPLC method for the determination of terazosin hydrochloride in human plasma.METHODS:Terazosin hydrochloride and internal standard(parazosin hydrochloride) were extracted from plasma with dichlormethane-diethylether(2:3) for determination.The separation was carried on a Diamonsil C18 column with the mobile phase consisted of 0.05mol· L-1 potassium dihydrogen phosphate buffer solution(pH4.8)-methanol-acetonitrile(61:25:14).The flow rate of the mobile phase was 1.0mL· min-1,and the excitation and emission wavelengths of the florescence det-ector were set at 334 and 385 nm,respectively.RESULTS:The linear range of terazosin hydrochloride was 0.25～ 64ng· mL-1(r=0.999 5),with the lowest detectable limit at 0.1ng· mL-1.The average extraction recovery was 84.0% and the average methodological recovery was 100.8%.The intra-day RSD and inter-day RSD were no more than 3.4% and 3.0%,espectively.CONCLUSION:This method is rapid,simple,accurate and sensitive,and suitable for the pharmacokinetic study and assaying of terazosin hydrochloride.