沙眼衣原体泌尿生殖道慢性持续感染患者人白细胞抗原DQA1基因多态性研究

目的 探讨人白细胞抗原DQA1 （HLA-DQA1）等位基因多态性与沙眼衣原体泌尿生殖道慢性持续性感染的相关性。方法 PCR和基因测序方法，对80例沙眼衣原体泌尿生殖道慢性持续感染患者、80例沙眼衣原体泌尿生殖道一般感染患者及80例正常人的HLA-DQA1等位基因进行检测。结果 HLA-DQA1*0102和DQA1*0501在沙眼衣原体泌尿生殖道慢性持续感染患者中的基因频率分别为22.5%、5.0%，在一般感染组的基因频率分别为5%、20%，而在正常人对照组的基因频率分别为2.5%、17.5%。沙眼衣原体泌尿生殖道慢性持续感染患者的HLA-DQA1*0102等位基因较一般感染组及正常人对照组增高，差异有统计学意义（χ2 = 14.6286，P < 0.01）；而HLA-DQA1*0501 等位基因在持续感染患者中下降，差异有统计学意义（χ2 = 6.2598，P < 0.05）。结论 HLA-DQA1*0102可能是沙眼衣原体泌尿生殖道慢性持续感染的易感基因或与易感基因相连锁。HLA-DQA1*0501等位基因可能具有阻止发生沙眼衣原体泌尿生殖道慢性持续感染的作用。

Polymorphism of HLA-D QA1 alleles in Chinese Han patients with persistent Chlamydia trachomatis genital infection

Objective To analyze the association of HLA-DQA1 gene polymorphism with persistent Chlamydia trachomatis genital infection. Methods Blood samples were collected from 80 patients with persistent genital Chlamydial infection，80 patients with common genital Chlamydial infection （who tested negative for Chlamydia trachomatis after one course of standard systemic treatment） and 80 normal human controls. HLA-DQA1 alleles were genotyped by PCR followed by gene sequencing. Results The frequency of HLA-DQA1*0102 allele and HLA-DQA1*0501 allele was 22.5% and 5.0% respectively in patients with persistent genital Chlamydial infection, 5% and 20% respectively in those with common genital Chlamydial infection, 2.5% and 17.5% respectively in normal human controls. Compared with the patients with common genital Chlamydial infection and controls, the patients with persistent genital Chlamydial infection had a higher frequency of HLA-DQA1*0102 （χ2 = 14.6286, P < 0.001）, but a lower frequency of DQA1*0501（χ2 = 6.2598, P < 0.05）. Conclusions HLA-DQA1*0102 allele may be a susceptible gene or closely linked with the susceptible genes of persistent genital Chlamydial infection. HLA-DQA1*0501 allele may have protective effects against persistent genital Chlamydial infection.