白血病病人异基因外周血干细胞移植后淋巴细胞TCRVβ基因片段的选择性扩增

目的:了解白血病病人异基因外周血干细胞移植后淋巴细胞TCRVβ基因片段选择性扩增的情况。方法:采用RT-PCR扩增5例CML和2例AML移植后患者外周血的单个核细胞的TCRVβ24个基因片段,分析其TCRVβ基因的表达情况。结果:不同标本中仅检测到2-8个VβT细胞基因片段,不同患者中缺乏的TCRVβ基因片段不尽相同。结论:移植后病人外周血淋巴细胞TCRVβ基因片段部分受抑制,部分基因片段呈选择性扩增。

Restricted expansion of T cell receptor (TCR)Vβ genes in leukemic patients subjected to allogeneic hematopoeitic stem cell transplantation

AIM: To investigate restricted expansion of TCR Vβ gene repertoire in patients with leukemia following allogeneic hematopoietic stem cell transplantation. METHODS: TCR Vβ subfamily genes in peripheral blood mononuclear cells from 7 cases of leukemia was amplified using RT-PCR. RESULTS: Only two-eight fragments of Vβ genes were detected in samples from these patients, and the detected fragments are different in different patients. CONCLUSION: TCR complexes were abnormal in all patients, part of the genes were seletively expansed and part of them were suppressed after transplantation.