我国雷氏按蚊和嗜人按蚊的分子鉴别和分类地位的探讨

目的 ]论证我国雷氏按蚊与嗜人按蚊的分类地位。 方法 ]采用分子鉴别法 (鉴别PCR和rDNA ITS2序列 )检测辽宁及山东两省现场按蚊标本 ,并依据ITS2区序列建立分子系统树。 结果 ]分子鉴别显示 ,辽宁和山东两省均存在雷氏按蚊和嗜人按蚊。我国雷氏按蚊 (辽宁和山东省 ,n =6 )和嗜人按蚊 (辽宁、云南、河南和四川省 ,n =10 )ITS2序列长度和GC含量分别为 45 1bp、 46 2 % ,44 8bp、 46 0 % ;各地雷氏按蚊和嗜人按蚊序列均无差异 ,但各地嗜人按蚊与对照组江苏的嗜人按蚊相比 ,种内序列差异为 0 88% ;雷氏按蚊与嗜人按蚊种间序列差异为 2 5 7%。分子系统树显示雷氏按蚊与中华按蚊、嗜人按蚊与凉山按蚊亲缘关系较近。 结论 ]根据分子鉴别 ,确认我国雷氏按蚊与嗜人按蚊为同域分布的 2个独立种

ON MOLECULAR IDENTIFICATION AND TAXONOMIC STATUS OF ANOPHELES LESTERI AND ANOPHELES ANTHROPOPHAGUS IN CHINA (DIPTERA:CULICIDAE)

Objective] To clarify the taxonomic status of Anopheles lesteri and An.anthropophagus in China. Methods] Using molecular identification (PCR assay and rDNA\|ITS2 sequencing) to examine the field anopheline mosquito specimens from Liaoning and Shandong. According to the ITS2 sequences, molecular phylogenetic tree was made. Results] According to the molecular identification, An.lesteri and An.anthropophagus were distributed both in Liaoning Province and Shandong Province. The length and GC content of rDNA\|ITS2 sequence were 451 bp, 46 2% in An.lesteri (n=6), and 448 bp, 46 0% in An.anthropophagus (n=10), respectively. The ITS2 sequences from presentation sites were same in An.lesteri, while the intraspecies difference in An.anthropophagus was 0 88%. The specific difference between An.lesteri and An.anthropophagus was 25 7%. By analyzing molecular phylogenetic tree, the relationship between An.lesteri and An.sinensis, An.anthropophagus and An.liangshanensis was found to be closer. Conclusion] According to the molecular identification, it was defined that An.lesteri and An.anthropophagus were sympatric independent species in China.