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| 断尾对小鼠血清褪黑素含量昼夜节律的影响 | |
| 引用本文： | 吴敏范,郭轶男,陈魁敏,商丽宏,郭丽,杨宇. 断尾对小鼠血清褪黑素含量昼夜节律的影响[J]. 中国医科大学学报, 2011, 40(8): 709-711 |
| 作者姓名： | 吴敏范 郭轶男 陈魁敏 商丽宏 郭丽 杨宇 |
| 作者单位： | 1. 沈阳医学院生理学教研室,沈阳,110034 2. 沈阳医学院机能实验中心,沈阳,110034 3. 沈阳医学院科学实验中心,沈阳,110034 |
| 摘 要： | 目的观察断尾对小鼠血清褪黑素含量昼夜节律的影响。方法分别随机将72 只正常雌性小鼠和72 只断尾（尾末端 2.5 cm 截断）雌性小鼠分成6 组，每组12 只，置于标准的明-暗周期下饲养2 周后，分别于4：00，8：00，12：00，16：00，20：00 及 24：00 迅速断头取血，用ELISA 法测定血清中褪黑素含量。结果正常小鼠血清褪黑素含量存在明显的昼夜节律，明期褪黑素 含量低，暗期褪黑素含量高，大约是明期的2.5 倍。断尾2 周后小鼠血清褪黑素含量也存在明显的昼夜节律，明期褪黑素含量 低，暗期褪黑素含量高，大约是明期的1.8 倍。结论正常小鼠与断尾2 周后小鼠血清褪黑素含量都存在明显的昼夜节律，与 正常小鼠相比，断尾2 周后小鼠明期血清褪黑素含量明显升高，并且波动较大。 |
| 关 键 词： | 褪黑素 昼夜节律 截肢 幻肢痛 小鼠 |
Effect of Amputating the Tail on the Circadian Rhythm of Serum Melatonin Level in Mice |
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| WU Min-fan , GUO Yi-nan , CHEN Kui-min , SHANG Li-hong , GUO Li , YANG Yu. Effect of Amputating the Tail on the Circadian Rhythm of Serum Melatonin Level in Mice[J]. Journal of China Medical University, 2011, 40(8): 709-711 | |
| Authors: | WU Min-fan GUO Yi-nan CHEN Kui-min SHANG Li-hong GUO Li YANG Yu |
| Affiliation: | WU Min-fan1,GUO Yi-nan1,CHEN Kui-min2,SHANG Li-hong2,GUO Li3,YANG Yu1(1.Department of Physiology,2.Center of Functional Experiment,3.Center of Scientific Experiment,Shenyang Medical College,Shenyang 110034,China) |
| Abstract: | Objective To investigate the effect of amputating the tail on the circadian rhythm of serum melatonin level in mice.Methods The distal 2.5 cm length of the tails in female mice were removed.72 normal and 72 tail-amputated female mice were divided into 6 groups randomly,with 12 mice in each group.All the mice were feeded under a standard light-dark rhythm(light phase 6:00-18:00;light:dark=12:12 h) for two weeks.Then they were decollated at 4:00,8:00,12:00,16:00,20:00 and 24:00,respectively,and the blood was ... |
| Keywords: | melatonin circadian rhythm amputation phantom limb pain mice |
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