肠炎宁颗粒对幼龄小鼠的抗炎止泻作用及体外抗菌作用研究

目的 研究肠炎宁颗粒作用于ICR幼龄小鼠的止泻抗炎药效及体外抑菌作用，为其临床儿童用药提供参考。方法 以ICR幼龄小鼠为考察对象，ig给予高、中、低剂量（2.500、1.250和0.625 g/kg）肠炎宁颗粒及蒙脱石散（阳性药，4.2 g/kg），连续给药3 d后，采用番泻叶浸出物和蓖麻油致泻模型、肠推进实验、冰醋酸致腹腔毛细血管通透性增加模型考察肠炎宁颗粒的止泻、抗炎作用；体外培养表皮葡萄球菌和大肠埃希菌，加入571.00、285.50、142.75、71.38、35.69、17.84、8.92、4.46、2.23和1.12 mg/mL的肠炎宁颗粒，观察其抑菌作用。结果 与模型组比较，0.625、1.250和2.500 g/kg的肠炎宁颗粒可以显著减少番泻叶浸出物、蓖麻油作用0~5 h内的腹泻样便数（P<0.05、0.001）；与对照组比较，2.5 g/kg的肠炎宁颗粒可以显著降低肠推进系数（P<0.05）；0.625、1.250和2.500 g/kg的肠炎宁颗粒可以显著减少冰醋酸所致的幼鼠腹腔毛细血管通透性增加（P<0.05、0.01）。8.92 mg/mL的肠炎宁颗粒即可在体外抑制表皮葡萄球菌和大肠埃希菌的生长，为最低抑菌浓度。结论 肠炎宁颗粒具有止泻、抑制肠胃推进及对抗急性渗出性炎症的作用；体外可以抑制表皮葡萄球菌和大肠埃希菌的生长。

Anti-diarrheal and anti-inflammatory effect and mechanism of Changyanning Granule in weaning mice

Objective The purpose of this article is to investigate the anti-diarrheal, anti-inflammatory and bacteriostatic effects of Changyanning granule in weaning mice, and provide reference for its pediatric use. Methods The ICR model of weaning mice was established and administrated with low, middle and high dose (0.625, 1.25, 2.50 g/kg) of Changyanning granuleand smecta (positive control, 4.20 g/kg). After continuous administration for three days, diarrhea models caused by castor oil and Senna Leaf extract, intestinal propulsion test and abdominal capillary permeability increase model caused by glacial acetic acid were used to test the antidiarrheal and anti-inflammatory effects of Changyanning granule. In vitro experiment was also performed to test the bacteriostatic effect of Changyanning granule. Staphylococcus epidermidis and Escherichia coli were cultured and treated with different concentrations of Changyanning granule (571.00, 285.50, 142.75, 71.38, 35.69, 17.84, 8.92, 4.46, 2.23, and 1.12 mg/mL). Results Compared with model group, diarrhea stool numbers were significantly reduced (P < 0.05, 0.001) after the dosing of Senna Leaf extract and castor oil within 5 h in 0.625, 1.25 and 2.50 g/kg Changyanning granule groups. Intestinal propulsion coefficient was reduced significantly (P < 0.05) in 2.50 g/kg Changyanning granule group in comparison with control group; the inhibitory actions of Changyanning (0.625, 1.25, 2.50 g/kg) on the increase of glacial acetic acid-induced abdominal capillary permeability were obviously (P < 0.05, 0.01); The growth of Staphylococcus epidermidis and Escherichia coli were inhibited with the initial concentration of 8.92 mg/mL in vitro. Conclusion Changyanning granule showed anti-diarrheal and acute exudative inflammation inhibition effects in weaning mice, and it could also inhibit the growth of Staphylococcus epidermidis and Escherichia coli in vitro.