川芎提取物通过激活Nrf2通路对抗心肌缺血大鼠氧化应激损伤

川芎是临床常用于心脑血管疾病的一味中药,现代医学证实川芎提取物具有良好的抗氧化活性。该文主要探讨川芎提取物对大鼠心肌缺血损伤氧化应激干预作用及可能的机制。采用皮下注射盐酸异丙肾上腺素诱导大鼠心肌缺血损伤模型,记录各组大鼠心脏指数,比色法检测血清肌酸激酶(CK)、乳酸脱氢酶(LDH)、谷草转氨酶(AST)、超氧化物歧化酶(SOD)、总抗氧化能力(T-AOC)的活性及血清中丙二醛(MDA)含量,同时光镜下观察心肌组织形态学变化,Western blot法检测心肌组织中核因子E2相关因子2(Nrf2)、醌氧化还原酶(NQO1)和血红素氧合酶-1(HO-1)蛋白表达的变化。结果显示,川芎提取物能显著降低心脏指数和血清心肌酶CK,LDH,AST水平,提高血清SOD和T-AOC活性并降低MDA含量,改善缺血损伤后心肌组织形态结构,增加心肌组织中Nrf2,NQO1,HO-1蛋白的相对表达。该研究表明川芎提取物对大鼠心肌缺血损伤具有保护作用,其机制可能与激活Nrf2信号通路,增加心肌组织抗氧化能力,抑制氧化应激反应有关。

Prevention effect of Ligusticum chuanxiong extraction against oxidative stress injury induced by myocardial ischemia through activation of Nrf2 signaling pathway

Ligusticum chuanxiong is one of the common traditional Chinese medicinal herbs for treating various cardiovascular and cerebrovascular diseases, and a number of previous studies have demonstrated that the extract of L. chuanxiong has strong antioxidative activity. This paper was mainly aimed to investigate the effects and possible mechanisms of L. chuanxiong extraction on oxidative stress induced by myocardial ischemia injury in rats. The rats were subcutaneously injected with isoprenaline hydrochloride to induce myocardial ischemia injury and treated for 2 weeks. Then the cardiac indexes of the rats were recorded. The concentration of malondialdehyde (MDA) and activities of serum creatine kinase (CK), lactate dehydrogenase (LDH), aspartate transaminase (AST), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) were measured by colorimetry. Light microscope was used to observe the morphological changes of myocardium, and the protein expressions of nuclear factor E2 related factor 2 (Nrf2), quinone oxidoreductase (NQO1) and hemeoxygenase-1 (HO-1) in cardiac tissue were evaluated by Western blot. The results showed that L. chuanxiong extraction could decrease cardiac indexes and the values of CK, LDH and AST in blood serum, increase activities of serum SOD and T-AOC, reduce serum MDA concentration, improve myocardium structure after ischemia injury, and up-regulate the protein expressions of Nrf2, NQO1 and HO-1 in cardiac tissue. These findings revealed that the cardioprotective effects of L. chuanxiong extraction may be related to inhibiting oxidative stress through the activation of Nrf2 signaling pathway.