川芎嗪、参麦注射液对缺氧复氧损伤后鼠脑星形胶质细胞释放一氧化氮的影响

目的：研究川芎嗪和参麦注射液对鼠脑星形胶质细胞缺氧复氧损伤后释放一氧化氮（nitric oxide,NO)的影响及可能机制。方法：培养Wistar乳鼠脑星形胶质细胞，用抗神经胶质纤维酸性蛋白单克隆抗体确认，建立缺氧／复氧模型，NO采用Griess反应法检测。结果：与对照组比较，缺氧复氧损伤后星形胶质细胞NO的释放显著增高，钙拮抗剂川芎嗪对缺氧复氧损伤后NO的过度释放无阻遏作用，而参麦注射液则能抑制O的过度分泌。结论：缺氧复氧损伤后星形胶质细胞释放NO增多可能与诱导型一氧化氮合成酶（iNOS)有关，参麦可能通过抑制NO的过度分泌以对抗缺氧复氧损伤。

The Effect of Tetramethylpyrazine and Shenmai Injection on Nitric Oxide Production in Cultured Cerebral Astrocytes of Rats with Damaging Produeced by Hypoxia Reoxygenation

Objective To study the effect and probable mechanism of tetramethylpyrazine and Shenmai injection on nitric oxide (NO) production in cultured cerebral astrocytes of rats with damaging produced by hypoxia reoxygenation.Methods The cerebral astrocytes of neonatal Wistar rats were cultured and were identified with anti-glial fibrillary acidic protein monoclonal antibody.The models of hypoxia reoxygenation were made among them.NO production was measured with the method of Griess.Result Compared with the control group,NO production increased rapidly after the astrocytes were damaged by hypoxia reoxygenation.Tetramethylpyrazine as calcium antagonist could not inhibit the over-abundant release of NO,but Shenmai injection could.Conclusion Astrocytes will product more nitric oxide than usual,when damaged by hypoxia reoxygenation.This may be related to the activity of iNOS.Shenmai injection can resist the hypoxia reoxygenation injury through inhibiting the abnormally high producting of NO.