白介素-10-627基因多态性和儿童哮喘的相关性研究

目的探讨白介素-10(IL-10)基因-627位点多态性与昆明地区儿童哮喘的相关性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法,检测50例哮喘儿童和36例健康儿童IL-10基因-627位点基因型,比较两组IL-10基因-627位点的基因型和等位基因分布频率。结果哮喘组和健康组3种基因型CC、CA、AA分别为:12.0%、48.0%、40.0%;8.3%、50.0%、41.7%。两组基因型分布差异无显著性(P>0.05)。哮喘组和健康组C和A的等位基因分布频率分别为:36.0%、64.0%;31.9%、68.1%。两组等位基因分布频率差异无显著性(P>0.05)。结论本研究表明IL-10基因-627位点多态性可能与昆明地区儿童哮喘易感性无关。

A study on the association between the interleukin-10-627(C/A) polymorphism and childhood asthma

Objective:To evalute the possible association between the IL-10-627 polymorphism and childhood asthma in Kunming.Methods:The study was conducted in two different groups: asthmatic children(n=50) and healthy children(n=32).The IL-10-627 polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism analysis(PCR-RFLP).Results: Frequencies of CC,CA and AA genotypes were 8.7%,48.0%,44.0% and frequencies of C,A allele were 32.0%,68.0% in the asthmatic group,however,frequencies of CC,CA and AA genotypes were 9.4%,46.9%,43.8% and frequencies of C,A allele were 32.8%,67.2% in the healthy group.There are no significant difference in the distribution of genotypes and allele frequencies between the two groups.Conclusion:Our data suggest that the IL-10-627 polymorphism may not be associated with childhood asthma in Kunming.