| 高效液相色谱法测定疏冠流浸膏在大鼠体内阿魏酸的含量 |
| |
| 引用本文： | 刘维佳,杨蕾,秦文娟.高效液相色谱法测定疏冠流浸膏在大鼠体内阿魏酸的含量[J].中国药学杂志,2001,36(5):339-340. |
| |
| 作者姓名： | 刘维佳 杨蕾 秦文娟 |
| |
| 作者单位： | 中国中医研究院西苑医院, |
| |
| 摘 要： | 目的探讨口服疏冠流浸膏在大鼠血清中阿魏酸的测定方法。方法用RP-HPLC测定疏冠流浸膏中阿魏酸的含量，条件：C18柱，以甲醇-1%醋酸水（35∶65）为流动相，紫外检测波长313nm。结果阿魏酸在40～200 μg·L-1浓度范围内回归方程为Y=5.15×10-6X+0.5824,相关系数r=0.9998。结论测定血清样品中阿魏酸用有机溶媒法较好。
|
| 关 键 词： | 高效液相色谱法 阿魏酸 血药浓度 |
| 文章编号： | 1001-2494(2001)05-0339-02 |
| 收稿时间： | 2000-05-18; |
| 修稿时间： | 2000年5月18日 |
Determination of ferulic acid in rat serum by HPLC after oral administration of
shu guan liquid extract |
| |
| LIU Wei jia,YANG Lei,QIN Wen juan.Determination of ferulic acid in rat serum by HPLC after oral administration of
shu guan liquid extract[J].Chinese Pharmaceutical Journal,2001,36(5):339-340. |
| |
| Authors: | LIU Wei jia YANG Lei QIN Wen juan |
| |
| Institution: | Department of Basic Medicine Science，Xiyuan Hospital，Chinese Academy of Traditional Chinese Medicine，Beijing 100091,China |
| |
| Abstract: | OBJECTIVE To develop an assay for the determination of ferulic acid in rat serum.METHODS The content of ferulic acid in serum after oral administration of Shuguan liquid extract was determined by HPLC.ODS C18column was used,the mobile phase was consisted of a mixture of methanol,1% acetic acid (35∶65 v/v).The detection wavelength was at 313 nm. RESULTS A linearity was obtained over the concentrations of 40～200μg·L-1(r=0.9998,The linear regression equation Y=5.15×10-6X+0.5824). CONCLUSION The determination method of ferulic acid in serum with organic solvent extraction was simple and accurate. |
| |
| Keywords: | HPLC ferulic acid serum |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 |
| 点击此处可从《中国药学杂志》下载免费的PDF全文 |
