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高效液相色谱法测定香连片中盐酸小檗碱含量
引用本文:黄玲,王明霞.高效液相色谱法测定香连片中盐酸小檗碱含量[J].中国现代药物应用,2010,4(13):15-16.
作者姓名:黄玲  王明霞
作者单位:济宁市中区妇幼保健院,272100
摘    要:目的建立香连片的盐酸小檗碱含量的测定方法。方法采用高效液相色谱法对方中的盐酸小檗碱进行含量测定,采用ODS色谱柱(250mm×4.6mm,5μm),柱温为室温,流动相为每100ml加十二烷基磺酸钠0.1g的乙腈旬.1%磷酸溶液(49:51),检测波长为265nm,流速:1ml/min。结果盐酸小檗碱进样量在1.95~13.0μg范围内线性良好,r=0.9995,平均回收率为98.84%(n=5),RSD=1.47%。结论试验结果表明本法简便快速,重现性好,结果准确可靠,可以作为香连片中盐酸小檗碱含量测定的方法。

关 键 词:高效液相色谱法  香连片  盐酸小檗碱  含量测定

Content of Berberine Hydrochloride in Xianglian Tablet by HPLC
HUANG Ling,WANG Ming-xia.Content of Berberine Hydrochloride in Xianglian Tablet by HPLC[J].Chinese Journal of Modern Drug Application,2010,4(13):15-16.
Authors:HUANG Ling  WANG Ming-xia
Institution:. The Woman and Child Health Hospital of The centre of fining fining Shandong,272100, China
Abstract:Objective To establish the method to determine the eontent of Berberine Hydroehloride in Xianglian Tablet. Methods The content of Berberine Hydrochloride in Xianglian Tablet was determined by HPLC, using ODS C18 column (250 mm ×4. 6 mm,5 μm) at 265 nm of detection wavelength, and at 25℃ ; the mobile phase was Acetonitrile -0. 1% Phosphate aeid (49:51) with 1.0 g/L Sodium Dodecyl Sulfate; The flow rate was 1. 0 ml/min. Results The linear ranges of Berberine Hydroehloride were 1.95 - 13.0μg, r = 0. 9995, the average recovery was 98.84% ( n = 5 ), and RSD was 1.47%. Conclusion The results indicated that the method is a simple, quick, well repeatable, correct and reliable way to determine the content of Berberine Hydroehloride in Xianglian Tablet, with well repeatability and eorreet results.
Keywords:HPLC method  Xianglian Tablet  Berberine Hydroehloride  Content determination
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