申克孢子丝菌基因差异、致病力与孢子丝菌病临床型别的关系

目的 探讨申克孢子丝菌基因差异、致病力与孢子丝菌病不同临床型别的关系。方法 ①收集不同临床型别孢子丝菌病的申克孢子丝菌分离株并提取DNA,进行随机扩增多态DNA(RAPD)扩增。②BALB/c小鼠接种不同临床型别孢子丝菌病的分离株菌悬液,观察实验动物发病及病变情况。③发病小鼠皮肤及内脏组织病理学检查,观察接种不同临床型别孢子丝菌病的分离株菌悬液后小鼠病变内申克孢子丝菌孢子数量及分布。结果 ①不同临床型别孢子丝菌病的申克孢子丝菌分离株聚合酶链反应产物电泳带型差异较明显:播散型分离株可见1800bp、850bp、500bp、180bp,皮肤淋巴管型分离株见1400bp、800bp、700bp、500bp,皮肤固定型分离株见2500bp、1400bp、1000bp、700bp。②注射播散型孢子丝菌病分离株菌悬液的BALB/c小鼠比注射皮肤淋巴管型分离株小鼠发病早、病变部位广且死亡率高;注射皮肤淋巴管型分离株的小鼠较注射固定型孢子丝菌病分离株小鼠皮损出现早、病变范围广且严重。③实验BALB/c小鼠病变皮肤及内脏组织病理学检查显示:注射播散型孢子丝菌病分离株的小鼠病变内孢子数量明显多于注射皮肤淋巴管型分离株小鼠病变内孢子数量,而后者较注射固定型孢子丝菌病分离株的小鼠病变内孢子数量多。结论 不同临床型别孢子丝菌病的申克孢子丝菌的基因差异、致病力与孢子丝菌病不同临床型别的关系密切。

Relationship of Genomic Characteristics, Virulence of Sporothrix Schenckii and Clinical Patterns of Sporotrichosis

Objective To explore the relationship of genomic characte ristics,virulence of S.schenckii and clinical patterns of sporotrichosis.Met hods Isolates of S.schenckii were collected from different clinical patterns of sporotrichosis.Genomic DNAs of S.schenckii isolates were amplified by rando mly amplified polymorphic DNA (RAPD).Suspensions of different isolates of S.sc henckii were inoculated into BALB/c mice in order to observe their pathogenicity.Histopathology of the skin and viscera of the mice was studied to determine th e numbers and distribution of spores.Results The electrophoretic profiles of PCR products differed in the isolates from different clinical patterns of sporo trichosis:1 800 bp,850 bp,500 bp,and 180 bp in disseminated sporotrichosis;1 400 bp,800 bp,700bp,and 500 bp in the regional lymphangitic sporotrichosis,2 500 bp,1400 bp,1000 bp,and 700 bp in fixed cutaneous sporotrichosis.The earl ier onset of illness,wider distribution of lesions and higher mortality rate we re observed in the BALB/c mice inoculated with isolates from disseminated sporot richosis,followed by those from regional lymphangitic sporotrichosis and those from fixed cutaneous sporotrichosis.Histopathologically more spores were found in the mice inoculated by isolates from disseminated sporotrichosis,followed by those from regional lymphangitic sporotrichosis and those from fixed cutaneous sporotrichosis.Conclusions There is a strong correlation among genomic charac teristics,virulence of S.schenckii and clinical patterns of sporotrichosis.