HPCE法测定卡马西平片的含量

目的：建立高效毛细管电泳（HPCE）法测定卡马西平片含量的方法。方法：采用熔融石英毛细管（27cm×50μm）作为分离通道；以30mmol·L^-1磷酸氢二钠溶液（pH=8．0）含75mmol·L^-1十二烷基硫酸钠（SDS）为运行缓冲液；分离电压18KV；毛细管柱温30℃，检测波长280nm。压力进样10s。结果：卡马西平在5～160μg·ml^-1浓度范围内线性关系良好（r=0．9997），平均回收率为99．9％，RSD5．8％。结论：方法简便、快速，可用于控制卡马西平片的质量。

Determination the Content of Carbamazepine Tablets by HPCE

Objective： To establish a HPCE method for determination the content of carbamazepine tablets. Method： The fusedsilica capillary（27cm ×50μm） was used, the separation voltage was 18kV, the column temperature was 30℃, the detection wavelength was 280nm, the running buffer solution was 30mmol·L^-1 disodium hydrogen Phosphate solution（ pH = 8.0） contain 75 mmol·L^-1 SDS, the sample was injected by pressure for 10s. Result： The linear range of carbamazepine was 5 - 160μg· ml^-1 （ r = 0.999 7 ）. The average recovery was 99.9% with RSD 5.8%. Conclusion： The method is simple and fast, It can be used for control the quality of carbamazepine tablets.