大肠癌患者外周血T细胞亚群和NK细胞活性检测的临床意义

目的 探讨大肠癌患者外周血T淋巴细胞亚群及NK细胞活性检测的临床意义。方法 采用SAP法及LDH释放法，对57例大肠癌患者和33例正常人的外周血T细胞亚群及NK细胞活性进行检测。结果 大肠癌患者CD4^ 细胞值、CD4^ /CD8^ 比值下降且NK细胞活性显著降低，CD8^ 细胞值显著高于对照组；这种变化随临床分期的增高更加显著（P＜0.05）；化疗后CD4^ 细胞值、CD4^＋／CD8^ 比值的下降和NK细胞活性的降低及CD8^＋细胞值的增高更为突出（P＜0.01）；术后发生转移的患者，CD4^＋细胞值、CD4^＋／CD8^＋比值下降及NK细胞活性下降，CD8^＋细胞值再回升。结论 大肠癌患者细胞免疫功能明显紊乱，并随病情进展及肿瘤转移而加重，切除肿瘤有助于改善患者细胞免疫功能，化疗时辅以免疫治疗是必要的。

Clinical Value of Detecting T Lymphocyte Subset and NK Cell Activity in Patients with Colorectal Cancer

Objective To study on the expression and clinical significance of T lymphocyte subset and NK cell activity(NKA) in patients with colorectal cancer.Methods 57 cancer patients and 33 healthy controls were enrolled in the study. T lymphocyte subset was measured by SAP techinique and NKA by a released LDH assay based on K 562 Cells,which served as target cells. Results The proportion of CD 4 cells,the ratio of CD 4/CD 8 and NKA of colorectal cancer patients markedly decreased compared with controls,while proportion of CD 8 cells significantly increased.Above change was closely related to the clinical stages of the tumor( P <0.05) and getting even more remarkably when chemotherapy was administered.Postoperatively,the proportion of CD 4 cells and the ratio of CD 4/CD 8,as well as NKA,increased significantly( P <0.01),while the proportion of CD 8 cells decreased. The results of dynamic observation were just contrary to above findings in patients with metastases.Conclusion Evidence suggests that obvious disorder of cellular immunologic function exists in patients with colorectal cancer and getting worse with the deveplopment and metastasis of tumor. Removal of tumor may be benefit to improve the cellular immunity of cancer patients.To enhance cellular immunologic function might be essential,especially when chemotherapy was administered.