3-MST mRNA在大鼠延髓的表达以及急性缺氧对其基因表达的影响

目的 探讨3-巯基丙酮酸硫转移酶(3-mercaptopyruvate sulfurtransferase,3-MST)mRNA在正常、急性缺氧及急性缺氧加外源性H2S条件下的表达情况.方法 21只SD大鼠随机等分为正常对照组、急性缺氧组和急性缺氧+ Na HS(H2S供体)组.建立大鼠急性缺氧模型,采用反转录聚合酶链反应(RT-PCR)技术,观察各组大鼠延髓3-MST mRNA的表达水平.结果 正常大鼠延髓有3-MST mRNA表达;给予急性缺氧刺激后,大鼠延髓3-MST mRNA表达量明显增加(P＜0.05);急性缺氧+NaHS组3-MST mRNA表达量比急性缺氧组明显减少(P＜0.05

Expression of 3-MST mRNA in Medulla Oblongata and Effects of Acute Hypoxia on Its Expression in Rats

Objective To investigate the expression of 3-Mercaptopyruvate sulfurtransferase（3-MST）in medulla oblongata of rats under normal, acute hypoxia and acute hypoxia plus exogenous hydrogen sulfide（H2S）. Methods 21 Sprage-Dawley rats were randomly divided into normal control,acute hypoxia and acute hypoxia plus sodium hydrosulfide（ NaHS）group. Acute hypoxia models of rats were established, then the expression levels of 3- MST mRNA were determined by RT-PCR in the medulla oblongata of each group rats. Results 3-MST mRNA were detected in the medulla oblongata of normal control rats,the expression levels of 3-MST mRNA were significantly increased in the rats with acute hypoxia（ P〈0.05 ）, whereas the expression levels of 3-MST mRNA were lower in acute hypoxia plus Naris group rats than that of acute hypoxia group（P〈0.05 ）. Conclusions The expression of 3- MST mRNA were existed in the medulla oblongata of normal rats. Acute hypoxia can up-regulated the expression of 3-MST mRNA in the medulla oblongata of rats. The increased expression caused by hypoxia-inducible of 3-MST mRNA can be restrained by adding exogenous H2 S. It suggests that H2 S produced under action of 3-MST in medulla oblongata may be involved in the respiratory response and protective effects of respiratory centers injury induced by acute hypoxia.