不同人群泌尿生殖道沙眼衣原体感染的分子流行病学研究

目的研究引起泌尿生殖道感染的沙眼衣原体流行基因型分布和不同人群间感染的差异。方法应用巢式PCR扩增沙眼衣原体omp1基因VS1-VS2序列,利用寡核苷酸芯片技术进行基因分型。结果166株沙眼衣原体感染标本中,通过基因分型共检出182株菌。包括9个基因型,总体优势流行型为E型(27.5%)、F型(22.0%)、D型(14.3%)、J型(15.0%)和H型(8.8%)。113例性病门诊病人中,以F(26.9%)、E(24.3%)、J(16.8%)、D(13.4%)和H(8.4%)型为主,混合型感染率为5.3%(6/113)。而53例卖淫女性标本中,发现混合感染8例(15.1%,8/53),以E(33.3%)、D(15.9%)、F(15.9%)和K(12.7%)型为主,混合感染率为15.1%(8/53)。F型在门诊病人中的流行率显著高于卖淫女性(!2=4.8,P<0.05),而混合型感染率和K型流行率卖淫女性显著高于门诊病人(!2=4.5,P<0.05;!2=7.4,P<0.01)。结论性病门诊患者和卖淫女性沙眼衣原体感染流行血清型存在差异。加强沙眼衣原体感染的分子流行病学研究对于有效控制性病传播有重要意义。

Epidemiological Study of Genital Chlamydia trachomatis Infection

Objective To study the distribution and occurrence of various serotypes of C.trachomatis. Method The VS1-VS2 of omp1 gene of C.trachomatis were amplified by nested PCR and genotyped by oligonucleotide array. Result Out of 166 C.trachomatis positive samples, 182 stains including 9 serovars were detected by the genotyping method. The most common serovars were serovar E (27.5%), F (22.0%), D (14.3%), J (15.0%) and H (8.8%). Among 113 patients attending the STDs clinic,serovar F(26.9%),E(24.3%),J(16.8%),D(13.4%)and H(8.4%) were the major serotypes, and 5.3% of patients had mixed infections. Among the 53 prostitutes, 8 had mixed infection, and serovar E(33.3%), D(15.9%),F(15.9%)and K(12.7%) were the major serotypes. Serovar F was most prevalent in patients attending the out patient clinic than the prostitutes(X2=4.8,P < 0.05). In constrast, serovar K and mixed infection was most prevalent among prostitutes (X2=4.5, P < 0.05; X2=7.4, P < 0.01). Conclusion Various serotypes of C.trachomatis were isolated from patients attending the STDs clinic and the prostitutes. Understanding the molecular epidemilogy of C.trachomatis infection is important in controlling this sexually transmitted disease.