| 雷公藤红素抑制血管生成的实验研究 |
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| 作者姓名： | Huang YL Zhou YX Zhou D Xu QN Ye M Sun CF Du ZW |
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| 作者单位： | 215006,苏州大学附属第一医院神经外科 |
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| 基金项目： | 江苏省非教育系统留学回国人员科技活动D类基金(1998年度 )资助项目 |
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| 摘 要： | 目的：探讨雷公藤红素对血管生成的抑制作用。方法：采用MTT法测定雷公藤红素对血管内皮细胞株(ECV)增殖的影响,观察雷公藤红素对ECV迁移实验和小管形成实验的作用,以及对鸡胚尿囊膜血管生成的影响。体内实验采用Matrigel plug方法。结果：雷公藤红素可明显抑制ECV的体外增殖,IC50为1．33μg／ml;可抑制ECV的迁移和小管形成,并且呈明显的剂量依赖性;同时具有抑制鸡胚尿囊膜血管生成和Matrigel plug中的血管新生的作用。结论：雷公藤红素具有明显抑制血管生成的作用,有可能成为有效的血管生成抑制剂。
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| 关 键 词： | 雷公藤红素 抑制 血管生成 实验研究 内皮细胞 抗肿瘤治疗 |
| 修稿时间： | 2002年9月19日 |
Celastrol in the inhibition of neovascularization |
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| Huang YL,Zhou YX,Zhou D,Xu QN,Ye M,Sun CF,Du ZW.Celastrol in the inhibition of neovascularization[J].Chinese Journal of Oncology,2003,25(5):429-432. |
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| Authors: | Huang Yu-lun Zhou You-xin Zhou Dai Xu Qi-nian Ye Ming Sun Cheng-fa Du Zi-wei |
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| Institution: | Department of Neurosurgery, First Hospital, Soochow University, Suzhou 215006, China. |
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| Abstract: | OBJECTIVE: To study the inhibition effect of celastrol on neovascularization. METHODS: The effect of celastrol on the in vitro proliferation of endothelial cell of vessel (ECV) was examined by MTT assay. The effect of celastrol on endothelial cell migration, tube formation on Matrigel and Chick chorioallantoic membrane angiogenesis was also examined. Matrigel plug assay was used to evaluate the effect of celastrol on angiogenesis in vivo. RESULTS: The proliferation of ECV was inhibited significantly by celastrol with IC(50) being 1.33 microg/ml. Celastrol inhibited endothelial cell migration and tube formation in a dose-dependent manner. Celastrol also inhibited angiogenesis both in Matrigel plug of mouse model and in chick chorioallantoic membranes. CONCLUSION: Celastrol, which can inhibit angiogenesis, could be developed as an antiangiogenic drug. |
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| Keywords: | Celastrol Angiogenensis Endothelial cell |
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