Enhanced antitumor effects by the coculture of allotumor RNA-pulsed dendritic cells with autologous cytokine-induced killer cells on hormone-refractory prostate cancer |
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Authors: | Pang Jun Gao Xin Liu Xiaopeng Wang Kebing Zhang Yan Feng Lianqiang Zhan Hailun |
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Institution: |
a Department of Urology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, China
b Department of Immunology, Basic Medical Institute of Sun Yat-sen University, Guangzhou, China |
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Abstract: | In this study, we evaluated antitumor effects of allotumour RNA-transfected dendritic cells (DCs) cocultured with autologous cytokine-induced killer cells (CIKs) on hormone-refractory prostate cancer. The cocultured cells enhanced prostate cancer cytolysis from 26% (CIKs-induced cytolysis) to 80.8%. They also increased the productions of CD4+ Th1 (IFN-γ+IL-4-, 55.52%) and CD8+ T (IFN-γ+, 69.59%) cells determined by intracellular cytokines IFN-γ /IL-4 staining and reduced the rate of CD4+ CD25+ cells from 18.72% (in CIKs) to 9.72%. The cocultured cells significantly inhibited tumor growth in SCID mouse and induced cancer cells necrosis and apoptosis. Our study indicates that tumor RNA-pulsed DCs cocultured with autologous CIKs significantly enhance antitumor immunity, which can be induced by increased CD4+ Th1 and CD8+ T cells and decreased CD4+CD25+ regulatory T (Treg) cells. This provides a potential immunotherapy strategy for HRPC. |
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Keywords: | dendritic cells cytokine-induced killer cells hormone-refractory prostate cancer immunotherapy |
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