兔视网膜中央静脉阻塞模型中小胶质细胞CD40和铁蛋白的表达

目的　分析兔视网膜中央静脉阻塞模型中小胶质细胞ＣＤ４０和铁蛋白表达的变化，探讨其发生机制。方法　将３０只有色兔随机分为空白组、模型组，以激光动力学方法造模，行眼底照相和眼底荧光血管造影法检查，分别于１ｄ、３ｄ、７ｄ、１４ｄ和２８ｄ对两组兔视网膜及视神经标本采用免疫组织化学方法检测ＣＤ４０和铁蛋白表达水平。４０倍光镜照相，测定灰度值和积分光密度（ＩＯＤ），对测得数据进行统计学分析。结果　小胶质细胞ＣＤ４０的表达结果:３ｄ时空白组和模型组视网膜标本灰度值分别为１９３．４２±９．７２和８６.０３±５．１２，差异有统计学意义（Ｐ＜０．０５）；３ｄ时空白组和模型组视神经标本灰度值分别为１９６．２６±１０．７８和８５．９１±５．０１，差异有统计学意义（Ｐ＜０．０５）；３ｄ时空白组和模型组视网膜标本ＩＯＤ分别为１０５．３６±７．５８和４４７９．３２±３０７．２２，差异有统计学意义（Ｐ＜０.０５）；３ｄ时空白组和模型组视神经标本ＩＯＤ分别为５４．６３±８．７９和１０４３６．４２±１３０６.８３，差异有统计学意义（Ｐ＜０．０５）。小胶质细胞铁蛋白的表达结果:３ｄ时空白组和模型组视网膜标本的ＩＯＤ分别为１６１．９６±３５．３７和１２９５．５９±１２４．７１，差异有统计学意义（Ｐ＜０．０５）。结论　视网膜中央静脉阻塞可以引起视网膜及视神经小胶质细胞的活化，免疫组织化学染色显示小胶质细胞ＣＤ４０和铁蛋白表达均为阳性结果。

Expression of microglia cells CD40 and ferritin in rabbit model of central retinal vein occlusion

Objective To analyze the change of the microglia cells CD40 and ferritin expression in the rabbit model of central retinal vein occlusion, and explore its mechanism. Methods Thirty colored rabbits were divided randomly into blank group and model group. The central retinal vein occlusion models were established with laser dynamics method, and then examined with fundus photography and FFA. The expression of CD40 and ferritin in retina and optic nerve at I day , 3 days,7 days , 14 days and 28 days were observed by immunohistochemistry. Photography was made with 40 times optical microscope , the gray value and IOD were exanuned. The data were analyzed by statistical software. Results The results of microglia CD40 expression : At 3 days , the gray values in retinal specimens of blank group and model group were 193. 42 +9. 72 and 86. 03 + 5 . 12 , respectively , the difference was statistically significant ( P < 0. 05 ) ; At 3 days,the gray values in optic nerve specimens of blank group and model group were 196. 26 + 10. 78 and 85. 91 + 5. 01 ,respectively , the difference was statistically sigruficant (P < 0. 05 ) ; At 3 days ,IOD in retinal specimens of blank group and model group were 105 . 36 + 7 . 58 and 4479. 32 + 307. 22 . respectively , the difference was statistically significant (P < 0. 05) ; At 3 days, IOD in optic nerve specimens of blank group and model group were 54. 63 + 8. 79 and 10 436. 42 + 1306. 83 , respectively , the difference was statistically significant ( P < 0. 05 ) . The results of microglia ferritin expression : At 3 days , IOD in retinal specimens of blank group and model group were 161. 96 + 35. 37 and 1295. 59 + 124. 71 . respectively , the difference was statistically sigruficant ( P < 0. 05 ) . Conclrision Central retinal vein occlusion can activate the microglia cells in retina and optic nerve. Microglia cells CD40 and Ferritin expression are positive in immunohistochemical study.