PI3K、p-Akt蛋白在喉鳞癌中的表达及临床意义

目的 研究磷脂酰肌醇3-激酶( phosphoinositide 3-kinase, PI3K) 和蛋白激酶B(protein kinase B, Akt)在人喉鳞状细胞癌组织中的表达特征及临床意义.方法 应用免疫组化S-P法检测40例喉鳞癌、20例声带息肉和20例喉乳头状瘤组织中PI3K和p-Akt的蛋白表达,显微镜下计数阳性细胞,对结果进行统计学分析.结果 PI3K和p-Akt在声带息肉组织中均呈低表达;在喉乳头状瘤中,PI3K高表达,而p-Akt呈低表达;在喉鳞状细胞癌组织中,PI3K和p-Akt均呈高表达.喉鳞状细胞癌中PI3K和p-Akt的表达与喉癌临床分期、颈部淋巴结转移及肿瘤的原发部位均无明显差异(P＞0.05).PI3K和p-Akt蛋白的阳性表达率随喉鳞癌分化程度的降低而降低,差异有统计学意义(P＜0.01).同一标本中PI3K和p-Akt的表达有显著的相关性(r=0.687, P＜0.01).结论 喉鳞癌存在PI3K和Akt蛋白的异常表达并与癌细胞的病理分级关系密切,PI3K/Akt途径的异常活化可能在喉鳞状细胞癌发生过程中起重要作用.

Expression and clinicopathologic significance of PI3K and p-Akt in laryngeal squamous cell carcinoma

Objective To investigate the expression of phosphoinositide 3-kinase (PI3K) and protein kinase B (Akt) and their role in pathogenesis in laryngeal squamous cell carcinoma. Methods Immunohistochemical S-P method was used to detect the expression of PI3K and p-Akt in 40 paraffin-embedded human laryngeal squamous cell carcinoma, 20 cases of polyp of vocal cords and 20 cases of laryngeal papilloma. All the samples were stained by HE method to confirm their pathologic characteristics. Positive rates of PI3K and p-Akt were evaluated by microscopy. Results PI3K and p-Akt showed low expression in the polyp tissues of vocal cords, while they were of significantly higher expression in laryngeal squamous cell carcinoma (P<0.01). Papilloma cells revealed a high expression of PI3K, but low expression of p-Akt. The poor differentiation of cancer cells correlated well with the low expression of PI3K and p-Akt (P<0.01).There was a positive relationship between PI3K and p-Akt in the same sample(r=0.687, P<0.01). Conclusion PI3K and p-Akt express highly in laryngeal squamous cell carcinomas, which is related to the pathological differentiation and pathogenesis of the tumor.