| 血清蛋白质组学研究中高丰度蛋白去除方法的建立 |
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| 引用本文： | 廖秋林,丁彦青,赵亮. 血清蛋白质组学研究中高丰度蛋白去除方法的建立[J]. 北京医学, 2007, 29(5): 293-296 |
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| 作者姓名： | 廖秋林 丁彦青 赵亮 |
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| 作者单位： | 广东省广州市南方医科大学病理学教研室,广东省分子肿瘤病理学重点实验室,510515;广东省广州市南方医科大学病理学教研室,广东省分子肿瘤病理学重点实验室,510515;广东省广州市南方医科大学病理学教研室,广东省分子肿瘤病理学重点实验室,510515 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)项目子课题，广东省科技攻关项目 |
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| 摘 要： | 目的 建立血清蛋白质组学研究中高丰度蛋白的去除方法并评价其去除效果.方法 应用Sigma公司2006年最新推出的血清白蛋白和IgG去除试剂盒(ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit)去除血清中高丰度的白蛋白和IgG等,再用双向凝胶电泳(2-DE)分析去除的效果.结果 本实验建立的血清中高丰度蛋白的去除方法,约95%的白蛋白和IgG能够被去除,2-DE图谱中蛋白质点更加清楚,部分低丰度蛋白得以显现.结论 成功建立血清中高丰度蛋白的去除方法.可为今后的血清蛋白质组学研究奠定基础.
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| 关 键 词： | 血清蛋白质组学 双向凝胶电泳 高丰度蛋白 白蛋白 |
| 修稿时间： | 2006-09-26 |
Establishment of method for removing high-abundance proteins serum proteomics research |
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| LIAO Qiu-lin,DING Yan-qing,ZHAO Liang. Establishment of method for removing high-abundance proteins serum proteomics research[J]. Beijing Medical Journal, 2007, 29(5): 293-296 |
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| Authors: | LIAO Qiu-lin DING Yan-qing ZHAO Liang |
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| Affiliation: | Department of Pathology, Southern Medical University, Guangdong Provincial Key Laboratory of Tumor Molecular Pathology, Guangzhou 510515 |
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| Abstract: | Objective To establish the method for removing high-abundance proteins in serum proteomics research. Methods Elimination of serum high-abundance proteins, such as albumin and IgG, was practiced by filtering with ProteoPrep Immunoaffinity Albumin and IgG Depletion Kit of Sigma company. The efficiency of purification was evaluated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Results The method for removing serum high-abundance proteins was established, about 95% albumin and IgG in serum could be removed. The protein dots of 2-DE map were more clear and some low-abundance proteins were visualization after filtering. Conclusions An effective method of removing high-abundance proteins in serum in serum is established. |
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| Keywords: | Serum proteomics Two-dimensional polyacrylamide gel electrophoresis (2-DE) High-abundance protein Albumin |
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