丁羟甲苯对紫外线致皮肤光老化的保护作用

目的：初步探讨丁羟甲苯（BHT）是否对紫外线（UV）所致皮肤光老化有保护作用。方法：利用无毛鼠动物模型模拟长期UV照射所致的皮肤光老化，采用病理组织切片制作技术，地衣红染色方法，40倍光镜观察真皮弹力纤维增生情况及生化分析方法测定真皮羟脯氨酸含量。结果：UV照射组大部分小鼠背侧表皮有皮肤光老化的明显特征，而BHT组小鼠皮肤状态无明显改变，对背部真皮弹力纤维增生的影响，BHT组与UV照射组比较差异有极显著性意义。与非UV照射线比较差异无显著性意义，而腹部真皮弹力纤维增生状况各组无差别，背部羟脯氨酸（HP）含量BHT组明显高于UV照射组，而腹部真皮羟脯氨酸含量各组均无差别，结论：在本实验条件下口服BHT对UV所致的无毛小鼠轻度皮肤光老化有保护作用。

Protective Effect of BHT on Skin Photoaging Induced by UV

Objective To preliminarily observe whether BHT has the protective effect on skin photoaging.Methods The hairless mice animal model are adopted,The hairless mice skin of back and ear are irradiated to simulate skin photoaging by long-term UV irradiation.Staining skin biopsies with orcein,under 40 times light microscopy,the grade of dermis elastic fiber hyperplasia are observed,while the changes of the contents of dermis hydroxyproline are measured by biochemical test method in each group of experiment.Results The mixing lamp with UVA and UVB could induce light skin photoaging of hairless mice.The condition of dermis elastic fiber hyperplasia in BHT UV group have prominent improvement compared with that in UV irradiation group.The contents of dermis hydroxyproline in BHT UV group are significantly higher than those in UV irradiation group.Conclusion Feeding BHT under the dose of this experimental study has the protective effect on light skin photoaging resulting from UV irradiation in hairless mice.