升麻中新三萜皂苷类成分研究

目的:开发利用升麻Cimicifuga foetida资源,从中寻找新的天然活性成分。方法:利用各种色谱技术进行分离纯化,根据理化性质和各种波谱技术辅以化学方法进行结构鉴定。结果:从升麻根茎80％的乙醇浸膏的EtOAc提取部分中分得4个升麻三萜皂苷类成分,分别鉴定为:7,8-二脱氢-27-脱氧升麻亭(7,8-didehydro-27-de-oxyactein,1),24-氧-乙酰升麻醇-3-O-β-D-木糖苷(23R,24R)[24-O-acetylshengmanol-3-O-β-D-xyl(23R,24R),2],升麻醇(cimigenol,3),升麻醇-3-O-β-D-木糖苷(cimigenol-3-O-β-D-xyl,4)。结论:化合物1为新化合物,2-4为首次从该植物中分得的化合物。体外抗骨质疏松药理活性筛选(以SRB法检测)结果表明,化合物1,2和4在浓度为10-9kg·L^-1时,对大鼠成骨肉瘤细胞株(URM106)有明显的增殖作用。

Studies on New Trierpenoid Constituents from the Rhizoma of Cimicifuga foetida

OBJECTIVE: To find new active constituents from Rhizome of Cimicifuga foetida. METHOD: Various column chromatographic techniques were employed for isolation and purification. The structures were elucidated on the basis of spectral and chemical evidences. RESULT: Four triterpenoid compounds were isolated and identified as 7,8-didehydro-27-deoxyactein(1), 24-O-acetylshengmanol-3-O-beta-D-xyl (23R, 24R)[2], cimigenol(3), cimigenol-3-O-beta-D-xyl(4). CONCLUSION: Compound 1 is a new compound, 2-4 were obtained from this medicinal material for the first time. The antiosteoporosis activity screening in vitro(by the method of SRB) indicates that Compounds 1, 2 and 4 can promote the proliferation for rat Osteoblastoma cell line (UMR106) at the concentration of 10(-9) kg.L-1.