芍药苷对黑素细胞生物活性的影响

目的:检测芍药苷对体外培养正常人表皮黑素细胞增殖活性、酪氨酸酶活性及黑素合成的影响。方法:建立正常人表皮黑素细胞培养体系,加入不同浓度(5~640μg/mL)芍药苷并作用一定时间后,采用MTT法、体外氧化DOPA反应法、NaOH法等分别测定黑素细胞增殖活性、酪氨酸酶活性及黑素含量的变化。结果:40~160μg/mL芍药苷呈剂量依赖性抑制黑素细胞增殖,与对照组比较差异有统计学意义(P0.05);5-20μg/mL芍药苷对酪氨酸酶活性呈剂量依赖性促进作用,与对照组比较差异有统计学意义(P0.05);10-20μg/mL芍药苷对黑素细胞黑素合成起促进作用,与对照组比较差异有统计学意义(P0.05)。结论:芍药苷在较高浓度范围内(40~160μg/mL)对体外培养正常人表皮黑素细胞增殖有剂量依赖性抑制作用;在较低浓度范围内(10-20μg/mL)对其黑素合成有促进作用。

Effect of paeoniflorin on biological activity in normal human malanocytes in vitro

Objective：To investigate the effect of paeoniflorin on cell proliferation,tyrosinase activity and melanin synthesis in cultured normal human melanocutes. Methods：After paeoniforin （5-640 μg/mL） was added into cultured normal human malanocytes,the effect of paeoniflorin on the proferation of melanocytes,ty-rosinase activity and synthesis of melanin in melanocytes were measured by MTT method,dopamine oxidation method and NaOH method,respectively. Results：40-160 μg/mL paeoniflorin inhibited the growth of mela-nocytes in a concentration-dependent manner （ P〈0.05） . When the concentrations of 5-20μg/mL paeonifor-in were added,the tyrosinase activity was significantly increased in a concentration-dependent manner （ P〈0.05）. When the concentrations of 10 -20μg/mL paeoniforin were added,the melanin synthesis were signifi-cantly increased （ P〈0.05） . The differences in the results mentioned above among the test groups and control group were significant （all Ps〈0.05）.Conclusion： Paeoniflorin in concentrations of 40-160 μg/mL can in-hibit the proliferation in cultured normal human malanocytes in a concentration-dependent manner. When the concentration of 10-20 μg/mL is used the melanin synthesis is promoted.