头痛宁胶囊对偏头痛模型大鼠中脑和下丘脑c-fos,c-jun基因表达的影响

目的:观察头痛宁胶囊对偏头痛模型大鼠中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun表达的影响,探讨头痛宁胶囊治疗偏头痛的作用机制。方法:健康SD大鼠随机分为:生理盐水组、偏头痛模型组、西比灵(1.04 mg·kg-1)治疗组、头痛宁高、中、低剂量(760,380,190 mg·kg-1)治疗组。sc硝酸甘油(NTG)制备偏头痛大鼠模型,给药后4,6 h 2个时间点取材,用免疫组化方法检测中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun表达水平。结果:与生理盐水组比较,模型组大鼠中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun阳性细胞数增多(P<0.01,P<0.01),4 h多于6 h。与模型组比较,头痛宁高、中剂量治疗组和西比灵治疗组大鼠中脑导水管周围灰质(PAG)和下丘脑c-fos,c-jun阳性细胞数减少(均P<0.01)。头痛宁低剂量治疗组无明显变化。结论:头痛宁胶囊可以减少由硝酸甘油诱导的致痛物质c-fos,c-jun的表达,从而抑制中脑导水管周围灰质(PAG)和下丘脑痛觉信息的传递,发挥治疗偏头痛的作用。其中,以头痛宁高、中剂量治疗组作用明显。

Effects of Toutongning Capsule on Expression of c-fos and c-jun in Mesencephalon and Thalamencephalon in Rats with Migraine

Objective: By observing the effects of Toutongning capsule on the expression of c-fos and c-jun in periaqueductalgrey (PAG) and thalamencephalon in rats with migraine to discuss the therapeutic mechanism of Toutongning capsule. Method: The SD rats were evenly divided into 6 groups:normal group,model group,Flunarizine(1.04 mg ·kg^-1)intervention group,high-dose,mid-dose and low-dose Toutongning capsule groups(760,380,190 mg ·kg^-1).Migraine model in rats was established by NTG subcutaneous injection.After NTG subcutaneous injection the specimen at 4 hours and 6 hours was obtained.The expression of c-fos and c-jun were detected by immunohistochemistry. Result: The expression of c-fos and c-jun in PAG and thalamencephalon were increased in the model group compared with that in the normal group (all P<0.01).At 4 hours after NTG subcutaneous injection,there was much more expression of c-fos and c-jun compared with those at 6 hours after NTG subcutaneous injection.The expressions of c-fos and c-jun in PAG and thalamencephalon were decreased in the high-dose Toutongning capsule intervention group,mid-dose Toutongning capsule intervention group and Flunarizine intervention group compared with those in low-dose Toutongning capsule group and in the model group (all P<0.01).There was no significant difference between model group and low dose intervention group. Conclusion: By reducing the expression of c-fos and c-jun and restrainning the transmission of pain information in PAG and thalamencephalon,Toutongning capsule could treat migraine.And the effect of high-dose Toutongning capsule and mid-dose Toutongning capsule were much better.