重庆产酸橙与甜橙枳实中挥发油成分的对比分析

目的:分析品种不同的枳实挥发油中的化学成分是否存在明显差异。方法:从重庆采集酸橙与甜橙枳实各2个批次,采用水蒸气蒸馏法提取挥发油,利用GC-MS联用技术进行成分分析,所得成分的质谱数据经数据库检索并与有关标准图谱资料核对鉴定,确定挥发油中的化学成分。应用色谱峰峰面积归一化法计算各成分的相对含量。结果:酸橙枳实挥发油鉴定了29个化学成分,甜橙枳实挥发油鉴定了38个化学成分,2个品种共有的化学成分24个,不同的化学成分19个,各成分含量有一定差异。同一品种的2个批次挥发油化学成分种类相同。结论:酸橙和甜橙枳实挥发油主要化学成分种类相同,但又存在一定差异。

Contrast Analysis of Chemical Constitutes in Volatile Oil of Citrus aurantium and Citrus sinensis from Chongqing

Objective: The volatile oils of Fructus Aurantii Immaturus are analyzed to verify whether the chemical constitutes are various in different species. Method: The Fructus Aurantii Immaturus samples of Citrus aurantium and Citrus sinensis are collected from Chongqing,and each species have two batches.The volatile oil which obtained by the method of water steam distillation are analyzed by GC-MS,then the chemical constitutes of volatile oil are identified by Data Base Retrieval and by comparing with the relative standard mass spectrum data,and the relative amount of each constitute is calculated by area normalization method. Result: Twenty-nine constitutes are identified in two batches of C. aurantium,and account for 97.4% in total oil.Thirty-eight constitutes are identified in two batches of C.sinensis, and account for 99.07% in total oil.Among them,twenty-four constitutes are common in the two species,and nineteen constitutes are different.Meanwhile,the content of each constitute exhibit some different between the two species.In the same species of Fructus Aurantii Immaturus,the chemical constitutes are no different in two batches. Conclusion: The chemical constitutes are obviously different between C.aurantium and C.sinensis.