风灵凝胶中秋水仙碱的体外透皮特性

目的:研究风灵凝胶中秋水仙碱的体外透皮吸收和体外释放情况。方法:采用改良Franz扩散池装置,以离体SD大鼠皮肤、透析膜为体外渗透屏障,采用HPLC测定接收液中秋水仙碱的含量,计算秋水仙碱单位面积累积渗透量和释药量,比较透皮速率和释放速率。结果:风灵凝胶中秋水仙碱8 h的体外释放和经皮渗透行为均符合Higuchi动力学方程,体外释药量高于经皮渗透量,且释药速率高于透皮速率。结论:皮肤为风灵凝胶中秋水仙碱透皮吸收的主要屏障,该制剂能平稳的释放药物,具有长效缓释的特点。

Study on in vitro Percutaneous Absorption Properties of Colchicine in Fengling Gelatin

Objective: To study the in vitro percutaneous absorption and in vitro release state of the main effective element colchicine in Fengling gelatin. Method: The Franz diffusion cells were chosen with excised SD rat skin and dialyzer as the in vitro permeability barrier; the content of colchicine was determined by HPLC, the cumulative permeation amount per unit area and release amount of colchicine were calculated, and the percutaneous absorption velocity and release velocity were compared. Result: The in vitro release and penetration process of colchicine in Fengling gelatin conformed to Higuchi kinetic equation process within 8h; the release amount of colchicine in Fengling gelatin was higher than cumulative permeation amount, and the release velocity was higher than percutaneous absorption velocity. Conclusion: Skin was the main percutaneous absorption barrier of colchicine in Fengling gelatin. The active constituent could be released steadily, which showed that Fengling gelatin was a prolonged action lente liberantes praeparatum.