健脾、温肾、疏肝、升清四法对IBS模型大鼠内脏敏感性及胃肠激素的作用研究

目的]观察健脾、温肾、疏肝、升清四法对IBS模型大鼠内脏敏感性及胃肠激素的影响.方法]采用脑-肠互动指向性条件应激IBS大鼠模型.将实验动物随机分为空白组、模型组、健脾组、温肾组、疏肝组、升清组,观察各组大鼠排便次数及性状、AWR评分变化、腹壁肌电活动、组胺体外释放量;血浆中胃动素（MOT）、神经肽Y（NPY）的含量,观察不同中医治法对内脏敏感性及胃肠激素的影响.结果]模型组排便次数及性状、AWR评分变化、腹壁肌电活动、组胺体外释放量与空白组相比,差异有显著意义（P＜0.05）,而各中药组与空白组相比,无明显差异（P＞0.05）,中药组之间亦无明显差异（P＞0.05）.同时模型组MOT含量显著高于空白组,且存在统计学差异（P＜0.01）,模型组NPY含量显著低于空白组,差异具有统计学意义（P＜0.01）;与模型组比较,温肾组、健脾组具有统计学差异（P＜0.05）,疏肝组也具有统计学差异（P＜0.01）;升清组与模型组差异无统计学意义（P＞0.05）;疏肝组、温肾组、健脾组组间差异不具有统计学意义.结论]健脾、温肾、疏肝、升清四法均能改善D-IBS大鼠肠道敏感性,其作用可能与降低AWR积分,抑制肥大细胞过量活化相关,同时,温肾组、健脾组、疏肝组对MOT及NPY具有有效的调节作用,而升清法未能体现有效的调节作用.

Four Methods of Strengthening Spleen, Warming Kidney, Relieving Liver and Clearing up Function to IBS Model Rats' Organs Sensitivity and Gastro-intestinal Hormone

Objective] To observe the influence of 4 methods on IBS model rats' internal organs sensitivity and gastro-intestinal hormone.Method] Randomly divide animals into groups of blank,model,spleen-strengthening,kidney-warming,liver-stretching and clearing up.Observe the secretion,AWR score,abdominal muscle movement,histamine burst size,MOT and NPY;observe the influence of different methods on viscera sensitivity and gastro-intestinal hormone.Result] Compared to blank group,the model group had marked differenc...