大鼠快肌与慢肌水溶性蛋白组的比较

目的 :对快肌和慢肌水溶性蛋白组进行比较 ,找出分别特异于快肌和慢肌的蛋白。方法 :应用蛋白组研究的核心技术———高分辨率二维电泳建立成年 (8月龄 )大鼠趾长伸肌 (代表快收缩纤维 )和比目鱼肌 (代表慢收缩纤维 )细胞内水溶性蛋白组表达的二维图谱。应用蛋白质氨基测序法和已知蛋白共电泳方法分别对经分离的蛋白进行鉴定。应用二维图谱差减法对快肌和慢肌的蛋白组进行比较。结果 :应用蛋白质氨基测序法和已知蛋白共电泳方法分别对 7个经分离的蛋白进行了鉴定。应用二维图谱差减法 ,发现了 3个分别特异于快肌和慢肌的蛋白。St1和St3仅在趾长伸肌被检测到 ,经蛋白质氨基测序法鉴定 ,St3为小钙白蛋白 ,而St2仅在比目鱼肌被检测到。结论 :快肌和慢肌细胞内水溶性蛋白组表达模式较为相似 ,但也分别存在其特异性蛋白 ,这些分别特异于快肌和慢肌的蛋白可能与其维持各自独特功能有关。小钙白蛋白和St1可作为鉴别快肌的标记物 ,而St2可作为鉴别慢肌的标记物。

Proteomics of Fast - twitch and Slow - twitch Skeletal Muscles

Objective To compare the aqueous proteomes of fast-twitch and slow-twitch muscles in order to screen proteins that are specific to fast-twitch and slow-twitch muscles respectively. Methods High resolution two-dimensional electrophoresis (2-DE) was used to generate the aqueous proteomes of extensor digitorum longus muscle (EDL, fast-twitch muscle) and soleus muscle (SOL, slow-twitch muscle) in adult rat (8 month-old). Protein N-terminal sequencing and known proteins co-migrating electrophoresis were applied to identify the isolated proteins in 2-DE gel. 2-DE patterns subtractive analysis was used to compare the protein profiles between fast- and slow-twitch muscles. Results Seven proteins that were isolated in 2DE gel were identified by protein N-terminal sequencing and known proteins co-migrating electrophoresis. In addition, three proteins (St1, St2 and St3) that were specific for EDL and SOL muscles were isolated respectively. St1 and St3 were only detected in EDL muscle, while St2 was only detected in SOL muscle. St3 is identified as parvalbumin by N-terminal sequencing. Conclusion Our results suggest that parvalbumin and St1 can be regarded as a marker of fast-twitch muscle, while St2 can be regarded as a marker of slow-twitch muscle. It is believed that these three proteins may play an important role in maintenance of physical function in their unique muscle fiber types respectively.