| 甘蔗渣多糖的纯化工艺 |
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| 引用本文： | 王许聪,刘莉,张璐,翁立冬,孙学刚,刘强.甘蔗渣多糖的纯化工艺[J].中国实验方剂学杂志,2010,16(14):31-34. |
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| 作者姓名： | 王许聪 刘莉 张璐 翁立冬 孙学刚 刘强 |
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| 作者单位： | 南方医科大学中医药学院中药制剂学教研室,广州,510515 |
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| 基金项目： | 广东省教育部产学研结合资助项目 |
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| 摘 要： | 目的:研究甘蔗渣中多糖的最佳纯化工艺。方法:用Savage法、TCA法脱蛋白;用双氧水法、活性炭法脱色;用AB-8,DB301,D101,MG-1大孔树脂纯化甘蔗渣多糖,用苯酚-硫酸法测定多糖的含量。结果:最佳工艺为用Savage法脱蛋白、活性炭脱色、MG-1型大孔吸附树脂纯化(上样量为0.3 g,柱高35 cm、流速10 mL/15 min),经富集后甘蔗渣多糖的含量由51.11%提高到了83.57%。结论:本实验为甘蔗渣多糖的分离纯化条件提供了参考,为甘蔗渣的进一步开发研究提供了依据。
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| 关 键 词： | 甘蔗渣多糖 脱蛋白 脱色 大孔吸附树脂 纯化工艺 |
| 收稿时间： | 2010/6/27 0:00:00 |
Study on Purification Process of Polysaccharides from Sugarcane Biogases |
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| WANG Xu-cong,LIU Li,ZHANG Lu,WENG Li-dong,SUN Xue-gang and LIU Qiang.Study on Purification Process of Polysaccharides from Sugarcane Biogases[J].China Journal of Experimental Traditional Medical Formulae,2010,16(14):31-34. |
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| Authors: | WANG Xu-cong LIU Li ZHANG Lu WENG Li-dong SUN Xue-gang and LIU Qiang |
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| Institution: | WANG Xu-cong,LIU Li,ZHANG Lu,WENG Li-dong,SUN Xue-gang,LIU Qiang(Department of Traditional Chinese Medicine of Nanfang Hospital,Southern Medical University,Guangzhou 510515,China) |
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| Abstract: | Objective:To explore the optimal purification process of the polysaccharides from sugarcane biogases.Method:To explore the optimum technology,we compared Savage method with TCA method for depro-teinization and hydrogen peroxide solution method with active carbon method for decoloration.And four common macroporous absorption resins were compared for differences in adsorption and desorption performance.Then phe-nol-sulfuric acid method was used to determine the content of the polysaccharides from sugarcane bi... |
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| Keywords: | polysaccharides from sugarcane biogases deproteinization decoloration macroporous absorp-tion resins purification process |
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