急性重复性缺氧小鼠脑组织中儿茶酚胺类神经递质含量变化的研究

用高效液相－电化学检测器测定急性重复性缺氧小鼠脑组织中ＤＯＰＡ、ＤＡ、ＮＥ和Ｅｐ等儿茶酚胺类物质的含量。结果发现：与空白对照组（Ａ组）比较，缺氧１次的实验对照组（Ｂ组）及急性重复缺氧４次实验组（Ｃ组），随缺氧次数增加，小鼠脑组织中的ＤＡ含量显著上升（Ｐ＜０．０１），ＮＥ、ＤＯＰＡ含量明显降低（Ｐ＜０．０５或＜０．０１）；Ａ组与急性重复４次缺氧后饲养２ｄ的实验对照组（Ｄ组）小鼠脑组织中儿茶酚胺类含量接近，没有统计学意义（Ｐ＝２３．７７）。结果提示脑组织中儿茶酚胺类物质可能参与急性重复缺氧小鼠的耐缺氧能力的形成，而且是一个可逆的快反应。

Studies of Catecholamine Transmitters in Acute Repetitive Hypoxia of Mouse Brain

Kunming mouse was chosen as the experimental animal. The levels of DOPA,DA,NE and EP in brain tissue were measured by high-performance liquid chromatography under acute repetitive hypoxia. As compared with the group A of blank control without exposed to hypoxia ,group B of experimental control was exposured to hypoxia only once ,and group D of another experimental control was exposed acutely and repetitively to hypoxia for 4 times and then fed for 2 days, the levels of DA increased significantly and that of DOPA,NE decreased markedly in experimental animals, group C ,acutely and repetitively exposed to hypoxia for 4 times; while the levels of catecholamines were similar between group A and D. There was no significant differences. The results indicated that catecholamines in brain might be involved in the development of tolerance in animals exposed to acute and repetitive hypoxia ,and the process might be a fast reversible response.