| HPLC法测定双黄连栓中绿原酸的含量 |
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| 引用本文： | 赵琪钟,李晓梅,李良昌. HPLC法测定双黄连栓中绿原酸的含量[J]. 中国药师, 2009, 12(6): 770-771 |
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| 作者姓名： | 赵琪钟 李晓梅 李良昌 |
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| 作者单位： | 普洱市食品药品检验所,云南,普洱,665000 |
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| 摘 要： | 目的：建立HPLC法测定双黄连栓中绿原酸含量。方法：色谱柱：Agilent ZORBAX Eclipse XDB—C18（150mm×4．6mm，5μm）柱；流动相：甲醇-水-冰醋酸（15：85：1）；流速：1．0ml·min^-1；检测波长：324nm。结果：绿原酸在0．077～0．387mg·ml^-1。范围内呈良好的线性关系（r=0．9999），平均回收率为99．9％（n=5），RSD：0．18％。结论：本法操作简单、灵敏、准确，可用于双黄连栓的质量控制。
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| 关 键 词： | 高效液相色谱法 双黄连栓 绿原酸 含量测定 |
Determination of Chlorophyll Acid in Shuanghuanglian Suppositories by HPLC |
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| Zhao Qizhong,Li Xiaomei,Li Liangchang. Determination of Chlorophyll Acid in Shuanghuanglian Suppositories by HPLC[J]. China Pharmacist, 2009, 12(6): 770-771 |
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| Authors: | Zhao Qizhong Li Xiaomei Li Liangchang |
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| Affiliation: | (Puer Institute for Food and Drug Control of Yunnan, Puer 665000, China) |
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| Abstract: | Objective： To establish the content determination method of chlorophyll acid in Shuanghuanglian suppositories by HPLC. Method： The column was used Agilent ZORBAX Eclipse XDB-Cls （150mm×4.6mm,5μm）. The mobile phase was methanol- water-acetic acid glacial（ 15： 85：1 ）. The flow rate was 1.0ml＇min -l. The detection wavelength was at 324 nm. Result： The linearity of chlorophyll acid was within the range of 0. 077 -0. 387ml·min^-1（ r = 0. 999 9 ）, and the average recovery was 99.9% （n = 5 ） （RSD = 0.18% ）. Conclusion： This method is simple, sensitive and accurate, and available for control the quality of Shuanghuanglian suppositories. |
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| Keywords: | HPLC Shuanghuanglian suppositories Chlorophyll acid Determination |
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